-glucosidase inhibitory and antioxidant activities of Entada spiralis Ridl. (Sintok) stem bark extracts

Opeyemi Roheem Fatimah, . and Siti Zaiton Mat So’ad, . and Uddin Ahmed Qamar, . and Norazian Mohd Hassan, . -glucosidase inhibitory and antioxidant activities of Entada spiralis Ridl. (Sintok) stem bark extracts. pp. 139-153. ISSN 1511-3701

Entada spiralis Ridl. (Leguminosae) locally known as Sintok or Beluru is a tropical woody climber that grows widely in Malaysia. It is a valuable and well-known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark by using petroleum ether chloroform and methanol as extracting solvents and were then bioassayed for their biological potential. The antioxidant and -glucosidase inhibitory activities of the extracts were assessed by using DPPH ABTS -carotene and -glucosidase inhibitory methods. Qualitative analysis showed the presence of most of the phytochemicals in methanol extract; however chloroform and petroleum ether extracts contained terpenoid and tannins as their major phytoconstituents respectively. The methanol extract contained the highest amount of total phenolics (42.5 15.85 g GAE/mg) and flavonoids (28.94 2.93 g QE/mg) and showed the most potent -glucosidase inhibitory activity with an IC‚‚ value of 20.67 g/mL. The same methanol extract exhibited the highest -carotene bleaching inhibition (27 at 1 mg/mL) while methanol and chloroform extracts exhibited good radical scavenging activities (IC‚‚ 37.29 0.05 and 90.84 3.12 g/mL respectively) against ABTS and DPPH radicals. Bioassay-guided silica gel column chromatography purification of the most active methanol extract afforded 3 4 5 7-tetrahydroxyflavone (6 mg). The compound displayed promising inhibitory activities against free radicals as well as -glucosidase enzyme. These results suggest the potential use of E. spiralis Ridl. stem bark as a therapeutic agent against hyperglycaemia.

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Abstract

Entada spiralis Ridl. (Leguminosae) locally known as Sintok or Beluru is a tropical woody climber that grows widely in Malaysia. It is a valuable and well-known plant in herbal medicine due to its various traditional and medicinal applications. Crude extracts were obtained from the stem bark by using petroleum ether chloroform and methanol as extracting solvents and were then bioassayed for their biological potential. The antioxidant and -glucosidase inhibitory activities of the extracts were assessed by using DPPH ABTS -carotene and -glucosidase inhibitory methods. Qualitative analysis showed the presence of most of the phytochemicals in methanol extract; however chloroform and petroleum ether extracts contained terpenoid and tannins as their major phytoconstituents respectively. The methanol extract contained the highest amount of total phenolics (42.5 15.85 g GAE/mg) and flavonoids (28.94 2.93 g QE/mg) and showed the most potent -glucosidase inhibitory activity with an IC‚‚ value of 20.67 g/mL. The same methanol extract exhibited the highest -carotene bleaching inhibition (27 at 1 mg/mL) while methanol and chloroform extracts exhibited good radical scavenging activities (IC‚‚ 37.29 0.05 and 90.84 3.12 g/mL respectively) against ABTS and DPPH radicals. Bioassay-guided silica gel column chromatography purification of the most active methanol extract afforded 3 4 5 7-tetrahydroxyflavone (6 mg). The compound displayed promising inhibitory activities against free radicals as well as -glucosidase enzyme. These results suggest the potential use of E. spiralis Ridl. stem bark as a therapeutic agent against hyperglycaemia.

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Item Type:	Article
AGROVOC Term:	Leguminosae
AGROVOC Term:	Woody plants
AGROVOC Term:	Herbaceous plants
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Phytochemistry
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Stems
AGROVOC Term:	Bark
AGROVOC Term:	Plant extracts
AGROVOC Term:	Phenolic content
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8128

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