PCR-RFLP characterization of major histocompatibility complex (MHC) B-LII gene in Nigerian locally adapted chickens

Bello-Ibiyemi Abdulraheem Adedeji, . and Adenaike Adeyemi Sunday, . and Ndubuisi Ikeobi Christian Obiora, . and Akpan Ubong, . and Takeet Michael Irewole, . PCR-RFLP characterization of major histocompatibility complex (MHC) B-LII gene in Nigerian locally adapted chickens. pp. 277-284. ISSN 1511-3701

DNA polymorphism at B-LII region of chicken Major Histocompatibility Complex (MHC) was studied in Nigerian locally adapted chicken genotypes namely: Normal-feathered Frizzled-feathered and Naked neck with much less history of selection for economic traits though previously selected for response to sheep red blood cell (SRBC). Response to SRBC (1 suspension i/v) was estimated by haemagglutination (HA) test 5 days post inoculation (dpi). Thereafter high and low responding groups were generated. DNA was isolated from high and low responders at second generation of selection as well as randomly selected individuals from the locally adapted chicken population. The B-LII was amplified with specific primers and an amplicon of 277 bp obtained in each sample was digested with three restriction enzymes (RE) viz. MspI BseRI and TaqI individually. While the PCRRFLP of B-LII with MspI and BseRI had no cutting sites TaqI RE exhibited monomorphic pattern with genotypes AA and at frequency 1.0 in the divergently selected groups. The Nigerian locally adapted chicken is known for high disease resistance. The monomorphic could be as a result of fixation of naturally selected genotype AA.

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Abstract

DNA polymorphism at B-LII region of chicken Major Histocompatibility Complex (MHC) was studied in Nigerian locally adapted chicken genotypes namely: Normal-feathered Frizzled-feathered and Naked neck with much less history of selection for economic traits though previously selected for response to sheep red blood cell (SRBC). Response to SRBC (1 suspension i/v) was estimated by haemagglutination (HA) test 5 days post inoculation (dpi). Thereafter high and low responding groups were generated. DNA was isolated from high and low responders at second generation of selection as well as randomly selected individuals from the locally adapted chicken population. The B-LII was amplified with specific primers and an amplicon of 277 bp obtained in each sample was digested with three restriction enzymes (RE) viz. MspI BseRI and TaqI individually. While the PCRRFLP of B-LII with MspI and BseRI had no cutting sites TaqI RE exhibited monomorphic pattern with genotypes AA and at frequency 1.0 in the divergently selected groups. The Nigerian locally adapted chicken is known for high disease resistance. The monomorphic could be as a result of fixation of naturally selected genotype AA.

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Item Type:	Article
AGROVOC Term:	Chickens
AGROVOC Term:	Major histocompatibility complex
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Restriction fragment length polymorphism
AGROVOC Term:	RFLP
AGROVOC Term:	Genotypes
AGROVOC Term:	DNA sequence
AGROVOC Term:	Genetic polymorphism
AGROVOC Term:	Disease resistance
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8133

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