Isolation and characterization of putative liver-specific enhancers in proboscis monkey (Nasalis larvatus)

Lim Leonard Whye Kit, . and Chung Hung Hui, . and Chong Yee Ling, . and Lee Nung Kion, . Isolation and characterization of putative liver-specific enhancers in proboscis monkey (Nasalis larvatus). pp. 627-647. ISSN 1511-3701

Enhancers are indispensable DNA elements responsible for elevation of gene transcriptional efficiency that regulates biological processes tightly at various developmental stages linking them to numerous genetic diseases. Discovering the enhancer landscape of the genome will not only benefit mankind but also aid in conservation researches involving endangered non-human primates such as the proboscis monkey. As one of the most ancient colobine endemic to Borneo Island the proboscis monkey offers a wide spectrum of unique and exclusive characteristics that distinguish it from other primates. This study has successfully isolated 13 liver-specific enhancers from this primate and tested for their activities in HepG2 and A549 cell line. The TFBS-enriched regions such as pairs of AP-1 clusters of C/EBP- and triplets of HNF-1 in enhancers contributed to enhancer activities whereas huge clusters of HNF-3 possess suppressing effects but generally these regions contributed to the cell specificities of enhancers. It is hoped that this study serves as a stepping stone in knowledge enrichment on this primate and future conservation researches.

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Abstract

Enhancers are indispensable DNA elements responsible for elevation of gene transcriptional efficiency that regulates biological processes tightly at various developmental stages linking them to numerous genetic diseases. Discovering the enhancer landscape of the genome will not only benefit mankind but also aid in conservation researches involving endangered non-human primates such as the proboscis monkey. As one of the most ancient colobine endemic to Borneo Island the proboscis monkey offers a wide spectrum of unique and exclusive characteristics that distinguish it from other primates. This study has successfully isolated 13 liver-specific enhancers from this primate and tested for their activities in HepG2 and A549 cell line. The TFBS-enriched regions such as pairs of AP-1 clusters of C/EBP- and triplets of HNF-1 in enhancers contributed to enhancer activities whereas huge clusters of HNF-3 possess suppressing effects but generally these regions contributed to the cell specificities of enhancers. It is hoped that this study serves as a stepping stone in knowledge enrichment on this primate and future conservation researches.

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Item Type:	Article
AGROVOC Term:	Primates
AGROVOC Term:	Monkeys
AGROVOC Term:	Isolation
AGROVOC Term:	Gene transcription
AGROVOC Term:	Computational biology
AGROVOC Term:	DNA sequence
AGROVOC Term:	Cloning
AGROVOC Term:	Liver
AGROVOC Term:	Wildlife conservation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8156

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