Genetic variation and relationship of NDama Cattle Toll-like receptor 5 with other bovine species

Durosaro Samuel Olutunde, . and Adepoju Babatunde Adetunji, . and Omotosho Israel, . and Oyaniyi Oluwatosin James, . and Ilori Babatunde Moses, . and Ajekigbe Sarah Oluwaseun, . and Taiwo Olumide Tobiloba, . Genetic variation and relationship of NDama Cattle Toll-like receptor 5 with other bovine species. pp. 647-667. ISSN 1511-3701

Toll-like receptor 5 is involved in innate immune responses that are initiated by host pattern recognition receptors (PRRs) which recognize molecular structures of conserved pathogen associated molecular patterns (PAMPs) expressed by microorganisms especially bacteria. In this study we sequenced 2577 bp bovine TLR5 in NDama cattle and discovered four synonymous mutations with one (C2127T) being shared between the NDama and the wild cattle. Sequences of other bovine species including Bos taurus Bos indicus and Bos javanicus from public domain revealed higher number of non-synonymous mutations 19 7 and 6 in wild cattle Bos indicus subspecies and the Bos taurus respectively with a higher ratio of total number of non-synonymous mutations to that of synonymous mutations suggesting that the gene is evolving under adaptive evolution. The results of genetic diversity revealed a combination of high haplotype diversity and low nucleotide diversity which is an evidence of past and rapid demographic expansion from a small effective population size. Haplotype reconstructions median-joining networks and phylogenetic analysis revealed haplotype sharing among Bos taurus Bos indicus and their hybrid suggesting retention of conserved ancestral variation that predates subspecies divergence in this immune gene. There is no haplotype sharing between the wild and the domestic cattle but a close relationship of the wild cattle clade with one of the NDama cluster suggesting little exchange of genetic material between these two groups of cattle. This results will facilitate effort towards understanding the relationship between mutations in different bovine species and their involvement in differential susceptibility and or tolerance to various diseases.

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Abstract

Toll-like receptor 5 is involved in innate immune responses that are initiated by host pattern recognition receptors (PRRs) which recognize molecular structures of conserved pathogen associated molecular patterns (PAMPs) expressed by microorganisms especially bacteria. In this study we sequenced 2577 bp bovine TLR5 in NDama cattle and discovered four synonymous mutations with one (C2127T) being shared between the NDama and the wild cattle. Sequences of other bovine species including Bos taurus Bos indicus and Bos javanicus from public domain revealed higher number of non-synonymous mutations 19 7 and 6 in wild cattle Bos indicus subspecies and the Bos taurus respectively with a higher ratio of total number of non-synonymous mutations to that of synonymous mutations suggesting that the gene is evolving under adaptive evolution. The results of genetic diversity revealed a combination of high haplotype diversity and low nucleotide diversity which is an evidence of past and rapid demographic expansion from a small effective population size. Haplotype reconstructions median-joining networks and phylogenetic analysis revealed haplotype sharing among Bos taurus Bos indicus and their hybrid suggesting retention of conserved ancestral variation that predates subspecies divergence in this immune gene. There is no haplotype sharing between the wild and the domestic cattle but a close relationship of the wild cattle clade with one of the NDama cluster suggesting little exchange of genetic material between these two groups of cattle. This results will facilitate effort towards understanding the relationship between mutations in different bovine species and their involvement in differential susceptibility and or tolerance to various diseases.

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Item Type:	Article
AGROVOC Term:	Bovines
AGROVOC Term:	Genetic variation
AGROVOC Term:	Phylogeny
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Immune response
AGROVOC Term:	Cattle
AGROVOC Term:	Species diversity
AGROVOC Term:	Disease resistance
AGROVOC Term:	Mutation
AGROVOC Term:	Susceptibility to infection
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8157

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