Histological observations of adventitious root derived from in vitro plantlet and shoot bud of Boesenbergia rotunda (Zingiberaceae)

Khairunnisa Abdul Ghani, . and Siti Zulaiha Ahamad Azhar, . and Nor Azma Yusuf, . Histological observations of adventitious root derived from in vitro plantlet and shoot bud of Boesenbergia rotunda (Zingiberaceae). pp. 699-707. ISSN 1511-3701

Boesenbergia rotunda or locally known as Temu kunci is renowned to possess some important bioactive compounds that are promising for pharmacological applications. It is essential to obtain structural information of the inner parts of the in vitro roots since growing these roots undertake various anatomical and morphological changes that influence their cells activities and nutrient uptake processes. Although root functions are thought to be significant for the growth of shoot the morphological and anatomical knowledge of adventitious root for this plant are limited. This particular study aims to investigate and compare anatomical structures of in vitro adventitious root derived from in vitro plantlet with root derived from shoot bud of B. rotunda. Histological sections using resin were done to study the anatomical of adventitious root of B. rotunda. The root samples were fixed in a Glutaraldehyde-Paraformaldehyde-Caffeine (GPC) fixative dehydrated infiltrated embedded cut stained and mounted with Surgipath mounting medium for observations under light microscope. From the histological observations adventitious roots of B. rotunda had shown the presence of all the three main tissue systems and had the same internal structures containing epidermis exodermis suberized sclerenchyma cells cortex and stele. Both adventitious roots derived from in vitro plantlet and shoot bud showed normal growth morphologically had anatomically same normal cell structures and arrangements.

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Abstract

Boesenbergia rotunda or locally known as Temu kunci is renowned to possess some important bioactive compounds that are promising for pharmacological applications. It is essential to obtain structural information of the inner parts of the in vitro roots since growing these roots undertake various anatomical and morphological changes that influence their cells activities and nutrient uptake processes. Although root functions are thought to be significant for the growth of shoot the morphological and anatomical knowledge of adventitious root for this plant are limited. This particular study aims to investigate and compare anatomical structures of in vitro adventitious root derived from in vitro plantlet with root derived from shoot bud of B. rotunda. Histological sections using resin were done to study the anatomical of adventitious root of B. rotunda. The root samples were fixed in a Glutaraldehyde-Paraformaldehyde-Caffeine (GPC) fixative dehydrated infiltrated embedded cut stained and mounted with Surgipath mounting medium for observations under light microscope. From the histological observations adventitious roots of B. rotunda had shown the presence of all the three main tissue systems and had the same internal structures containing epidermis exodermis suberized sclerenchyma cells cortex and stele. Both adventitious roots derived from in vitro plantlet and shoot bud showed normal growth morphologically had anatomically same normal cell structures and arrangements.

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Item Type:	Article
AGROVOC Term:	Zingiberaceae
AGROVOC Term:	Tissue analysis
AGROVOC Term:	Microscopy
AGROVOC Term:	Adventitious roots
AGROVOC Term:	Shoots
AGROVOC Term:	In vitro plantlets
AGROVOC Term:	Cell structure
AGROVOC Term:	Plant morphology
AGROVOC Term:	Plant anatomy
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8160

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