Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability

Alexander Miatong, . and Nur Faizah Abu-Bakar, . and Chong Elizabeth Sinirisan, . and Siti Shahara Zulfakar, . and Anith Amirah Aidilputra, . Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability. pp. 709-726. ISSN 1511-3701

Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count APC Enterobacteriaceae Escherichia coli E. coli and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.771.14 log CFU/cm (meansd). Enterobacteriaceae was found on 75 of the samples at 3.311.14 log CFU/cm. E. coli was only detected on 11.36 of the total contact surfaces at 2.911.00 log CFU/ cm whereas 25 of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms especially at 4C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count APC Enterobacteriaceae Escherichia coli E. coli and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.771.14 log CFU/cm (meansd). Enterobacteriaceae was found on 75 of the samples at 3.311.14 log CFU/cm. E. coli was only detected on 11.36 of the total contact surfaces at 2.911.00 log CFU/ cm whereas 25 of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms especially at 4C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.

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Item Type:	Article
AGROVOC Term:	Abattoirs
AGROVOC Term:	Beef
AGROVOC Term:	Food contamination
AGROVOC Term:	Microbial contamination
AGROVOC Term:	Slaughtering
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Pathogenic microorganisms
AGROVOC Term:	Enterobacteriaceae
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Salmonella
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8161

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