Detection of sheep poxvirus by nested-polymerase chain reaction assay

Sharif S., . and Ghorashi S. A., . Detection of sheep poxvirus by nested-polymerase chain reaction assay. pp. 7-12. ISSN 9128-2506

Sheep pox disease is one of the most important diseases caused by poxviruses in animals. This disease can pose an economic threat to animal husbandry in countries where the virus has not been eradicated. In this study a rapid PCR assay was optimised and developed for the detection of sheep poxvirus. The target region was the inverted terminal repeats (ITRs) of the viral genome and the primers used were able to replicate a product of 289 bp. The primers detected the virus from skin biopsies of sheep suspected of poxvirus infection and the identity of PCR products was confirmed by sequencing which showed 97 homology to published sequences. The primers also work well in detecting a vaccine strain of goat poxvirus. The sensitivity of the assay improved 10 times by using nested-PCR assay. Detection of sheep poxvirus by PCR in clinical samples has many advantages compared with other methods that are currently being used. The assay proved to be sensitive specific and rapid. Therefore it can be used as a robust diagnostic test in veterinary laboratories.

Full text available from: Official URL: https://www.mavma.org.my/2010-volume-22-issue-no-1...

Abstract

Sheep pox disease is one of the most important diseases caused by poxviruses in animals. This disease can pose an economic threat to animal husbandry in countries where the virus has not been eradicated. In this study a rapid PCR assay was optimised and developed for the detection of sheep poxvirus. The target region was the inverted terminal repeats (ITRs) of the viral genome and the primers used were able to replicate a product of 289 bp. The primers detected the virus from skin biopsies of sheep suspected of poxvirus infection and the identity of PCR products was confirmed by sequencing which showed 97 homology to published sequences. The primers also work well in detecting a vaccine strain of goat poxvirus. The sensitivity of the assay improved 10 times by using nested-PCR assay. Detection of sheep poxvirus by PCR in clinical samples has many advantages compared with other methods that are currently being used. The assay proved to be sensitive specific and rapid. Therefore it can be used as a robust diagnostic test in veterinary laboratories.

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Item Type:	Article
AGROVOC Term:	Sheep
AGROVOC Term:	Diagnosis
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Identification
AGROVOC Term:	Animal viruses
AGROVOC Term:	Animal diseases
AGROVOC Term:	Infection
AGROVOC Term:	Vaccines
AGROVOC Term:	Lesions
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8229

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