The relevance of viral culture based methods for the detection of rat cytomegalovirus (RCMV ALL-03) in the modern era of technology

Zuridah Hassan, . and Nazariah Allaudin Zeenathul, . and Siti Nazrina Camalxaman, . and Mohd Azmi Mohd Lila, . and Quah Yi Wan, . and Loh Hwei San, . and Sheikh Omar Abdul Rahman, . The relevance of viral culture based methods for the detection of rat cytomegalovirus (RCMV ALL-03) in the modern era of technology. pp. 16-21. ISSN 9128-2506

In spite of the unequivocal advantages of traditional virological methods its utilization seems to have taken a back seat coincident with the concurrent advances in science and technology. This study examined the impact of such techniques in the groundwork identification of rat cytomegalovirus ALL-03 strain. Virus was validated in terms of its intrauterine novelty cytopathogenicity and was identified based on morphologic criteria classical of herpesvirus. Our findings demonstrate the suitability of primary brain endothelial cells for the propagation of the virus resulting in concomitant production of cytopathic effects and formation of plaques comparable to the ones produced in established secondary cell lines. In the absence of any known sequences for molecular based detection method such findings provided critical clues crucial for the preliminary identification of the virus. This paper concludes that conventional methods such as tissue culture and electron microscopy are still in the forefront in the field of virology and remains relevant but should ideally be used in conjunction with more advanced methodologies for a more comprehensive evaluation of novel viruses.

Full text available from: Official URL: https://www.mavma.org.my/2011-volume-23-issue-no-2...

Abstract

In spite of the unequivocal advantages of traditional virological methods its utilization seems to have taken a back seat coincident with the concurrent advances in science and technology. This study examined the impact of such techniques in the groundwork identification of rat cytomegalovirus ALL-03 strain. Virus was validated in terms of its intrauterine novelty cytopathogenicity and was identified based on morphologic criteria classical of herpesvirus. Our findings demonstrate the suitability of primary brain endothelial cells for the propagation of the virus resulting in concomitant production of cytopathic effects and formation of plaques comparable to the ones produced in established secondary cell lines. In the absence of any known sequences for molecular based detection method such findings provided critical clues crucial for the preliminary identification of the virus. This paper concludes that conventional methods such as tissue culture and electron microscopy are still in the forefront in the field of virology and remains relevant but should ideally be used in conjunction with more advanced methodologies for a more comprehensive evaluation of novel viruses.

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Item Type:	Article
AGROVOC Term:	Cytomegalovirus
AGROVOC Term:	Virology
AGROVOC Term:	Identification
AGROVOC Term:	Herpesvirus
AGROVOC Term:	Tissue culture
AGROVOC Term:	Electron microscopy
AGROVOC Term:	Pathogenicity
AGROVOC Term:	Host pathogen systems
AGROVOC Term:	Herpesviridae
AGROVOC Term:	Rats
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8395

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