Citation
Mat Yunus, Abdul Masani and Ghulam Kadir, Ahmad Parveez (2003) Construction of transformation vectors for synthesizing biodegradable plastics in the mesocarp of transgenic oil palm. [Proceedings Paper]
Abstract
Production of biodegradable plastic, polyhydroxybutyrate (PHB), involves three steps; condensation of two molecules of acetyl-CoA to yield acetoacetyl-CoA by β-ketothiolase (encoded by the gene phbA or bktB), reduction of acetoacetyl CoA to (R)-3-hydroxybutyryl-CoA by acetoacetyl-CoA reductase (encoded by the gene phbB) and polymerization of (R)-3-hydroxybutyryl-CoA to yield PHB by PHB synthase (encoded by the gene phbC). In addition, for the production of co-polymer, polyhydroxybutyrate-co¬velerate (P HBV), an initial step for the production of substrate propionyl CoA is required through the introduction of threonine dehydratase enzyme encoded by tdcB gene. In this study, we have proposed to synthesize PHB and PHBV in transgenic oil palm, specifically in the mesocarp tissues. We have successfully engineered two vectors (pMS29 and pMS31) carrying PHB and PHBV genes, respectively, driven by oil palm mesocarp-specific promoter (MSP 1). Plastid targeting sequence (transit peptide;TP) has been fused to 5'end of the open reading frame of each target genes to ensure the expression is targeted in the plastid. Matrix attachment region of tobacco (RB7-MAR) was also included, with the hope of stabilizing transgene expression and minimizing silencing due to position effect. Transformation of the vectors into oil palm embryogenic calli has just been initiated.
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Abstract
Production of biodegradable plastic, polyhydroxybutyrate (PHB), involves three steps; condensation of two molecules of acetyl-CoA to yield acetoacetyl-CoA by β-ketothiolase (encoded by the gene phbA or bktB), reduction of acetoacetyl CoA to (R)-3-hydroxybutyryl-CoA by acetoacetyl-CoA reductase (encoded by the gene phbB) and polymerization of (R)-3-hydroxybutyryl-CoA to yield PHB by PHB synthase (encoded by the gene phbC). In addition, for the production of co-polymer, polyhydroxybutyrate-co¬velerate (P HBV), an initial step for the production of substrate propionyl CoA is required through the introduction of threonine dehydratase enzyme encoded by tdcB gene. In this study, we have proposed to synthesize PHB and PHBV in transgenic oil palm, specifically in the mesocarp tissues. We have successfully engineered two vectors (pMS29 and pMS31) carrying PHB and PHBV genes, respectively, driven by oil palm mesocarp-specific promoter (MSP 1). Plastid targeting sequence (transit peptide;TP) has been fused to 5'end of the open reading frame of each target genes to ensure the expression is targeted in the plastid. Matrix attachment region of tobacco (RB7-MAR) was also included, with the hope of stabilizing transgene expression and minimizing silencing due to position effect. Transformation of the vectors into oil palm embryogenic calli has just been initiated.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number |
| AGROVOC Term: | oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis |
| AGROVOC Term: | biodegradable plastics |
| AGROVOC Term: | genetic transformation |
| AGROVOC Term: | mesocarp |
| AGROVOC Term: | genetic engineering |
| AGROVOC Term: | polymerase chain reaction > polymerase chain reaction Prefer using PCRPCR |
| AGROVOC Term: | plasmids |
| AGROVOC Term: | transgenic plants |
| AGROVOC Term: | biodegradable plastics |
| AGROVOC Term: | environmental conditions > environmental conditions Prefer using environmental factorsenvironmental factors |
| Geographical Term: | Malaysia |
| Depositing User: | Nor Hasnita Abdul Samat |
| Date Deposited: | 23 Jul 2024 02:13 |
| Last Modified: | 23 Jul 2024 02:13 |
| URI: | http://webagris.upm.edu.my/id/eprint/842 |
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