Limited immune response conferred by an E2-CSFV expressing DNA vaccine after booster vaccination in murine models

Sheik Omar A. R., . and Mohd Azmi M. L., . and Bahaman A. R., . and Aini I., . and Zeenathul N. A., . and A. R. Mutalib, . Limited immune response conferred by an E2-CSFV expressing DNA vaccine after booster vaccination in murine models. pp. 1-7. ISSN 9128-2506

To investigate if intradermal (ID) and intramuscular (IM) routes of vaccination result in a comparable expression of E2 gene of classical swine fever virus (CSFV) an established E2 DNA construct was tested in murine experimental models. Groups of six mice received three pcDNAE2 immunisation each at weeks 0 4 and 8 by either ID via gene gun delivery or IM. Another second batch of six mice was immunised in a similar protocol with a plasmid cocktail of pcDNAE2 and immunomodulators (pBOOST-mIL4/Mil13). The findings showed that the IM route was better in immune induction compared to the gene gun method. Apparently on the basis of E2 expression in skin tissues via ID ear targeting seemed to be better than the abdominal skin approach. With respect to the kinetics of anti-E2 antibody production the antibody was not formed after one and two months after the DNA immunisation but reached its detection level at 10 weeks post-immunisation. Variations were observed in the ability of the E2 expression plasmids to induce immune response in murine model when the humoral and cell-mediated immunity (CMI) was determined by ELISA and DTH trials respectively. The adopted ID and IM immunisation approaches gave distinct immune induction patterns in the presence and absence of cytokines. The findings indicate the enhancement of immune response after co-administration of these genetic immunomodulators with DNA vaccine against CSFV. However the difference was insignificant (P0.05) due to the high variance caused by the presence of non-responsive individuals.

Full text available from: Official URL: https://www.mavma.org.my/2009-volume-21-issue-no-2...

Abstract

To investigate if intradermal (ID) and intramuscular (IM) routes of vaccination result in a comparable expression of E2 gene of classical swine fever virus (CSFV) an established E2 DNA construct was tested in murine experimental models. Groups of six mice received three pcDNAE2 immunisation each at weeks 0 4 and 8 by either ID via gene gun delivery or IM. Another second batch of six mice was immunised in a similar protocol with a plasmid cocktail of pcDNAE2 and immunomodulators (pBOOST-mIL4/Mil13). The findings showed that the IM route was better in immune induction compared to the gene gun method. Apparently on the basis of E2 expression in skin tissues via ID ear targeting seemed to be better than the abdominal skin approach. With respect to the kinetics of anti-E2 antibody production the antibody was not formed after one and two months after the DNA immunisation but reached its detection level at 10 weeks post-immunisation. Variations were observed in the ability of the E2 expression plasmids to induce immune response in murine model when the humoral and cell-mediated immunity (CMI) was determined by ELISA and DTH trials respectively. The adopted ID and IM immunisation approaches gave distinct immune induction patterns in the presence and absence of cytokines. The findings indicate the enhancement of immune response after co-administration of these genetic immunomodulators with DNA vaccine against CSFV. However the difference was insignificant (P0.05) due to the high variance caused by the presence of non-responsive individuals.

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Item Type:	Article
AGROVOC Term:	Vaccines
AGROVOC Term:	Immune response
AGROVOC Term:	Vaccination
AGROVOC Term:	Gene expression
AGROVOC Term:	Swine fever virus
AGROVOC Term:	Immunization
AGROVOC Term:	Plasmids
AGROVOC Term:	Antibodies
AGROVOC Term:	Immunity
AGROVOC Term:	ELISA
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8422

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