Citation
Noor Azmi Shaharuddin and Ahmad Parveez Ghulam Kadir (2003) Isolation of RNA from oil palm tissues for screening of constitutively expressing genes in oil palm. [Proceedings Paper]
Abstract
Like many genetically engineered plants, transgenic oil palms (Elaeis guineensis) have been made using constitutive promoters to drive its transgenes. In this study, the aimed is to isolate and characterize constitutively expressing genes from oil palm. The genes will later be used to isolate their regulatory elements from genomic libraries. Several standard methods for plant RNA isolation were applied on different tissues of oil palm and the protocol that gave the best yield was identified. RNA prepared by this method shows no degradation and free from contaminating DNA. Typical yields of the RNA ranged from 70-600 µg/g fresh weight of tissue, which compares favorably with yields reported for other methods designed for high-phenolic plants. Total RNA from young leaves, mature leaves, flowers, roots, fronds, mesocarp and kernels at different stages of development, have been obtained by this method. The extracted RNA is suitable for applications such as mRNA isolation and dot blot hybridization. Towards accomplishing the study, cDNA clones that shows non¬differential expression based on the microarray approach were screened against the mRNAs of oil palm to identify clones that are expressed in every tissue of oil palm. Results obtained to date and problems faced during the study will be elaborated.
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Abstract
Like many genetically engineered plants, transgenic oil palms (Elaeis guineensis) have been made using constitutive promoters to drive its transgenes. In this study, the aimed is to isolate and characterize constitutively expressing genes from oil palm. The genes will later be used to isolate their regulatory elements from genomic libraries. Several standard methods for plant RNA isolation were applied on different tissues of oil palm and the protocol that gave the best yield was identified. RNA prepared by this method shows no degradation and free from contaminating DNA. Typical yields of the RNA ranged from 70-600 µg/g fresh weight of tissue, which compares favorably with yields reported for other methods designed for high-phenolic plants. Total RNA from young leaves, mature leaves, flowers, roots, fronds, mesocarp and kernels at different stages of development, have been obtained by this method. The extracted RNA is suitable for applications such as mRNA isolation and dot blot hybridization. Towards accomplishing the study, cDNA clones that shows non¬differential expression based on the microarray approach were screened against the mRNAs of oil palm to identify clones that are expressed in every tissue of oil palm. Results obtained to date and problems faced during the study will be elaborated.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number |
| AGROVOC Term: | oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis |
| AGROVOC Term: | RNA |
| AGROVOC Term: | gene expression |
| AGROVOC Term: | molecular biology |
| AGROVOC Term: | data analysis |
| AGROVOC Term: | sampling |
| AGROVOC Term: | isolation |
| AGROVOC Term: | molecular markers > molecular markers Prefer using genetic markersgenetic markers |
| AGROVOC Term: | plant biotechnology |
| Geographical Term: | Malaysia |
| Depositing User: | Nor Hasnita Abdul Samat |
| Date Deposited: | 03 Aug 2024 06:48 |
| Last Modified: | 03 Aug 2024 06:48 |
| URI: | http://webagris.upm.edu.my/id/eprint/847 |
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