Detection of Avian Metapneumovirus (AMPV) field infection via reverse transcriptase polymerase chain reaction (RT-PCR) and ELISA in two layer farms in Johor

Seetha J., . and Abu J., . and Goh Y. M., . and Lim A. A. S., . and Choo P. Y., . Detection of Avian Metapneumovirus (AMPV) field infection via reverse transcriptase polymerase chain reaction (RT-PCR) and ELISA in two layer farms in Johor. pp. 9-13. ISSN 9128-2506

Avian Metapneumovirus (AMPV) infection which is known as 'swollen head syndrome' has been shown to be prevalent in poultry farms in Malaysia. Two layer farms in Johor denoted as Farm A and Farm B with previous history of AMPV disease outbreak were the subjects used for the AMPV field investigation in this study. Thirty chicks from respective treatment groups were monitored at day old two four and six weeks of age for AMPV antibody and antigen detection. RT-PCR and ELISA serology indicate that at 2 weeks of age AMPV field infection had occurred in Farm A. In Farm B AMPV field infection or lateral spread of vaccine virus was observed as early as 2 weeks. AMPV seroconversion was generally observed at four weeks of age and Al\1PV subtypes A and B were detected via RT-PCR from both farms in this study. This is the first report of AMPV subtypes A and B by RT-PCR detection in Malaysia.

Full text available from: Official URL: https://www.mavma.org.my/2009-volume-21-issue-no-1...

Abstract

Avian Metapneumovirus (AMPV) infection which is known as 'swollen head syndrome' has been shown to be prevalent in poultry farms in Malaysia. Two layer farms in Johor denoted as Farm A and Farm B with previous history of AMPV disease outbreak were the subjects used for the AMPV field investigation in this study. Thirty chicks from respective treatment groups were monitored at day old two four and six weeks of age for AMPV antibody and antigen detection. RT-PCR and ELISA serology indicate that at 2 weeks of age AMPV field infection had occurred in Farm A. In Farm B AMPV field infection or lateral spread of vaccine virus was observed as early as 2 weeks. AMPV seroconversion was generally observed at four weeks of age and Al\1PV subtypes A and B were detected via RT-PCR from both farms in this study. This is the first report of AMPV subtypes A and B by RT-PCR detection in Malaysia.

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Item Type:	Article
AGROVOC Term:	Livestock farms
AGROVOC Term:	Chickens
AGROVOC Term:	Poultry
AGROVOC Term:	Animal diseases
AGROVOC Term:	Bird diseases
AGROVOC Term:	Disease outbreak
AGROVOC Term:	Serology
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	ELISA
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8470

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