Citation
A. R. Mutalib, . and Phumoonna T., . and A. R. Bahaman, . and Mohd Azmi M. L., . Leptospiral infection in stray dogs in Malaysia. pp. 23-27. ISSN 9128-2506
Abstract
Leptospirosis is a zoonotic disease of worldwide importance. Besides rodents dogs have also been implicated as a source of infection to humans. Samples of urine and blood from 142 stray dogs were examined for leptospirosis by serological test and bacterial cultures. Of these 83 (118/142) and 33 (47/142) of the serum samples were positive by ELISA-IgM/IgG and the microscopic agglutination test (MAT) respectively. No leptospiral isolates were obtained on culture. Leptospira interrogans serovar pomona was found to be the most predominant serovar in the dogs with an infection rate of 68 at MAT titres of 100. Urine and serum samples were randomly selected for detection and identification of leptospires by the polymerase chain reaction (PCR) and low “stringency PCR assay respectively. All seropositive samples selected were positive by PCR assay. One urine samples showed the presence of leptospires based on PCR assay.
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Abstract
Leptospirosis is a zoonotic disease of worldwide importance. Besides rodents dogs have also been implicated as a source of infection to humans. Samples of urine and blood from 142 stray dogs were examined for leptospirosis by serological test and bacterial cultures. Of these 83 (118/142) and 33 (47/142) of the serum samples were positive by ELISA-IgM/IgG and the microscopic agglutination test (MAT) respectively. No leptospiral isolates were obtained on culture. Leptospira interrogans serovar pomona was found to be the most predominant serovar in the dogs with an infection rate of 68 at MAT titres of 100. Urine and serum samples were randomly selected for detection and identification of leptospires by the polymerase chain reaction (PCR) and low “stringency PCR assay respectively. All seropositive samples selected were positive by PCR assay. One urine samples showed the presence of leptospires based on PCR assay.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Dogs |
| AGROVOC Term: | Infection |
| AGROVOC Term: | Leptospirosis |
| AGROVOC Term: | Serological diagnosis |
| AGROVOC Term: | Microbial culture |
| AGROVOC Term: | Urine |
| AGROVOC Term: | Blood |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Enzyme linked immunosorbent assay |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8478 |
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