Molecular and fatty acid analyses of transformed oil palm cultures and plantlets


Citation

Salmah Yaakop and Bahariah Bohari and Sabariah Kamis and Ahmad Parveez Ghulam Kadir (2003) Molecular and fatty acid analyses of transformed oil palm cultures and plantlets. [Proceedings Paper]

Abstract

Putative transgenic embryoids and plantlets carrying antisense palmitoyl-ACP thioesterase gene were proven via PCR analysis. Internal control of oil palm were used to optimise PCR condition. After optimization, DNA were subjected to PCR amplifications using Basta resistant gene and followed by antisense palmitoyl-ACP thioesterase gene. The antisense palmitoyl-ACP thioesterase gene was expected to change the fatty acid composition (%) by decreasing the palmitic acid and hopefully increasing the oleic acid. The functionality of the transgene is being determined via Gas Chromatography (GC) analysis.


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Abstract

Putative transgenic embryoids and plantlets carrying antisense palmitoyl-ACP thioesterase gene were proven via PCR analysis. Internal control of oil palm were used to optimise PCR condition. After optimization, DNA were subjected to PCR amplifications using Basta resistant gene and followed by antisense palmitoyl-ACP thioesterase gene. The antisense palmitoyl-ACP thioesterase gene was expected to change the fatty acid composition (%) by decreasing the palmitic acid and hopefully increasing the oleic acid. The functionality of the transgene is being determined via Gas Chromatography (GC) analysis.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number
AGROVOC Term: oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis
AGROVOC Term: fatty acids
AGROVOC Term: transgenic plants
AGROVOC Term: data analysis
AGROVOC Term: sampling
AGROVOC Term: plantlets (seedlings) > plantlets (seedlings) Prefer using seedlingsseedlings
AGROVOC Term: genes
AGROVOC Term: gene expression
AGROVOC Term: genetic transformation
Geographical Term: Malaysia
Depositing User: Nor Hasnita Abdul Samat
Date Deposited: 03 Aug 2024 07:37
Last Modified: 03 Aug 2024 07:37
URI: http://webagris.upm.edu.my/id/eprint/848

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