Transfer of PHB and PHBV genes into oil palm for the production of biodegradable plastics

Ahmad Parveez Ghulam Kadir and Abdul Masani Mat Yunus and Salmah Yaakop and Bahariah Bohari and Sabariah Kamis and York, Gregory and Yeong, Bae Jo (2003) Transfer of PHB and PHBV genes into oil palm for the production of biodegradable plastics. [Proceedings Paper]

Oil palm is an important economic crop for Malaysia. Genetic engineering could be applied to produce transgenic oil palms with high value fatty acids and novel value-added products to ensure the sustainability of the palm oil industry. One of the targets of the oil palm genetic engineering programme is synthesizing biodegradable plastics (polyhydroxyalkanoates) in oil palm. Two types of plastics targeted were polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-velerate (PHBV). Few constructs for producing the above plastics were produced and transformed into oil palm. Two plasmids, produced by MIT scientist, which carries all the genes under the control of ubiquitine promoter, were transformed into oil palm embryogenic calli and have to date produced many putative transgenic poliembryogenic lines and small plantlets. PCR and Gas Chromatography analyses on the transformed cultures and plantlets have been initiated with some encouraging results. Transformation of oil palm embryogenic calli with four other constructs (two each for PHB and PHBV driven by either combination of constitutive promoters or mesocarp specific promoter), produced at MPOB, has been initiated with some has already undergoing selection on Basta. Progress made to date and problem faced will be discussed.

Full text available from:

Abstract

Oil palm is an important economic crop for Malaysia. Genetic engineering could be applied to produce transgenic oil palms with high value fatty acids and novel value-added products to ensure the sustainability of the palm oil industry. One of the targets of the oil palm genetic engineering programme is synthesizing biodegradable plastics (polyhydroxyalkanoates) in oil palm. Two types of plastics targeted were polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-velerate (PHBV). Few constructs for producing the above plastics were produced and transformed into oil palm. Two plasmids, produced by MIT scientist, which carries all the genes under the control of ubiquitine promoter, were transformed into oil palm embryogenic calli and have to date produced many putative transgenic poliembryogenic lines and small plantlets. PCR and Gas Chromatography analyses on the transformed cultures and plantlets have been initiated with some encouraging results. Transformation of oil palm embryogenic calli with four other constructs (two each for PHB and PHBV driven by either combination of constitutive promoters or mesocarp specific promoter), produced at MPOB, has been initiated with some has already undergoing selection on Basta. Progress made to date and problem faced will be discussed.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number
AGROVOC Term:	oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis
AGROVOC Term:	biodegradable plastics
AGROVOC Term:	Polyhydroxybutyrate-co-valerate
AGROVOC Term:	polymerase chain reaction > polymerase chain reaction Prefer using PCRPCR
AGROVOC Term:	data analysis
AGROVOC Term:	transgenic plants
AGROVOC Term:	genes
AGROVOC Term:	gene expression
AGROVOC Term:	biotechnology
AGROVOC Term:	genetic engineering
Geographical Term:	Malaysia
Uncontrolled Keywords:	Polyhydroxybutyrate, Polyhydroxybutyrate-co-valerate
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	03 Aug 2024 07:42
Last Modified:	03 Aug 2024 07:42
URI:	http://webagris.upm.edu.my/id/eprint/849

Actions (login required)

View Item