Transformation of oil palm with antisense Palmitoyl-ACP thioesterase gene for increasing oleic acid content

Ahmad Parveez Ghulam Kadir and Abrizah Othman and Ahmad Tarmizi Hashim and Zamzuri Ishak and Ahmad Kushairi Din and Salmah Yaakop and Bahariah Bohari and Sabariah Kamis (2003) Transformation of oil palm with antisense Palmitoyl-ACP thioesterase gene for increasing oleic acid content. [Proceedings Paper]

Oil palm is an important economic crop for Malaysia. Genetic engineering could be applied to produce transgenic oil palms with high value fatty acids and novel value-added products to ensure the sustainability of the palm oil industry. The main target of the oil palm genetic engineering programme is increasing oleic acid content at the expense of palmitic acid. A construct carrying antisense copy of the oil palm palmitoyl-ACP thioesterase gene driven by a constitutive promoter has been used to transformed oil palm embryogenic calli derived from solid and liquid cultures. The construct also carries a Basta resistant gene for selection of trans formants. The bombarded embryogenic calli were transformed and selected on medium containing Basta. Resistant embryogenic calli obtained were subjected to full plant regeneration. Some of the full-grown plantlets have been transferred onto soil and grown in the biosafety nursery. Molecular and fatty acid analyses on some of the cultures have been initiated. Progress made to date and problem faced will be discussed.

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Abstract

Oil palm is an important economic crop for Malaysia. Genetic engineering could be applied to produce transgenic oil palms with high value fatty acids and novel value-added products to ensure the sustainability of the palm oil industry. The main target of the oil palm genetic engineering programme is increasing oleic acid content at the expense of palmitic acid. A construct carrying antisense copy of the oil palm palmitoyl-ACP thioesterase gene driven by a constitutive promoter has been used to transformed oil palm embryogenic calli derived from solid and liquid cultures. The construct also carries a Basta resistant gene for selection of trans formants. The bombarded embryogenic calli were transformed and selected on medium containing Basta. Resistant embryogenic calli obtained were subjected to full plant regeneration. Some of the full-grown plantlets have been transferred onto soil and grown in the biosafety nursery. Molecular and fatty acid analyses on some of the cultures have been initiated. Progress made to date and problem faced will be discussed.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number
AGROVOC Term:	oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis
AGROVOC Term:	oleic acid
AGROVOC Term:	fatty acids
AGROVOC Term:	polymerase chain reaction > polymerase chain reaction Prefer using PCRPCR
AGROVOC Term:	data analysis
AGROVOC Term:	polyembryony
AGROVOC Term:	transgenic plants
AGROVOC Term:	gene expression
AGROVOC Term:	genetic transformation
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	03 Aug 2024 07:51
Last Modified:	03 Aug 2024 07:51
URI:	http://webagris.upm.edu.my/id/eprint/850

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