Citation
Samsul Kamal Rosli and N., Gorret and Tarmizi A.H. and Cheah, S. C. and Sinskey, A. J. and Rha, C. K. and Badlishah Sham Baharin and Mohd Azmuddin Abdullah (2003) Utilisation of carbon and nitrogen sources by oil palm cultures in the bioreactor. [Proceedings Paper]
Abstract
Bioreactors have been widely used for the industrial production of microbial, animal and plant metabolites. Use of a bioreactor system for palm suspension cultures can provide quantitative data on oil palm suspension cells since control of environmental variables, such as temperature, mixing, and amount of dissolved oxygen can be easily maintained in a bioreactor. The bioreactor study was initiated at MIT using the Sixfors Multi Fermenter System (Infors, Bottmingen, Switzerland). During the culture of oil palm suspensions in a batch bioreactor, nutrients and extracellular metabolites were monitored to characterize the behavior of oil palm suspension cultures. It was found that the carbon source, sucrose, was hydrolyzed to glucose and fructose within the first 10 days and both were completely utilized by the 25th day. Nitrogen sources from the MS L-media remained in excess until the end of the growth period while only 30% of ammonia and 15% of nitrates were consumed. However, the results showed that different ortets respond differently to the growth conditions. This study provides a better understanding of oil palm suspension culture with regards to the growth, nutrient uptake and metabolite production. This information will further enhance the progress of oil palm clonal materials development towards mass propagation.
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Abstract
Bioreactors have been widely used for the industrial production of microbial, animal and plant metabolites. Use of a bioreactor system for palm suspension cultures can provide quantitative data on oil palm suspension cells since control of environmental variables, such as temperature, mixing, and amount of dissolved oxygen can be easily maintained in a bioreactor. The bioreactor study was initiated at MIT using the Sixfors Multi Fermenter System (Infors, Bottmingen, Switzerland). During the culture of oil palm suspensions in a batch bioreactor, nutrients and extracellular metabolites were monitored to characterize the behavior of oil palm suspension cultures. It was found that the carbon source, sucrose, was hydrolyzed to glucose and fructose within the first 10 days and both were completely utilized by the 25th day. Nitrogen sources from the MS L-media remained in excess until the end of the growth period while only 30% of ammonia and 15% of nitrates were consumed. However, the results showed that different ortets respond differently to the growth conditions. This study provides a better understanding of oil palm suspension culture with regards to the growth, nutrient uptake and metabolite production. This information will further enhance the progress of oil palm clonal materials development towards mass propagation.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number |
| AGROVOC Term: | oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis |
| AGROVOC Term: | carbon |
| AGROVOC Term: | nitrogen |
| AGROVOC Term: | data analysis |
| AGROVOC Term: | fermentation |
| AGROVOC Term: | bioreactors |
| AGROVOC Term: | nutrient uptake |
| AGROVOC Term: | biomass production |
| Geographical Term: | Malaysia |
| Depositing User: | Nor Hasnita Abdul Samat |
| Date Deposited: | 03 Aug 2024 08:05 |
| Last Modified: | 03 Aug 2024 08:05 |
| URI: | http://webagris.upm.edu.my/id/eprint/851 |
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