Production of carotenoids by Rhodotorula toruloides isolated from Brazilian tropical savannah

Silva L. G., . and Machado W. R. M., . and Vanzela E. S. L., . and Del Bianchi V. L., . Production of carotenoids by Rhodotorula toruloides isolated from Brazilian tropical savannah. pp. 1259-1267. ISSN 2231-7546

Several artificial colouring agents are used as food additives to improve the foods visual appearance. A recent increase in the use of natural colouring with bioactive properties (antioxidants) as a substitute for additives used in the food industry has led to the search for novel sources to produce such substances with functional colouring. The present work was aimed to isolate and select yeasts from the Cerrado (Savannah) biome (Central region Brazil) to produce carotenoids. Sixty-nine of the 470 colonies selected after screening presented colours from yellow to red. These yeasts were grouped into three colours: yellow pink and orange. Yeast belonging to the pink group identified as Rhodotorula toruloides was chosen for improvement of the factors (physical and nutritional) involved in submerged cultivation. Carotenoid bioproduction was improved by using an experimental design which evaluated the characteristics of the physical processes (agitation and temperature) followed by 2�� factorial experimental design to select the relevant factors for the culture medium. Following statistical analysis a complete second-order experimental design was employed to optimise the composition of the culture medium. The maximum carotenoid production obtained was 1 333.11 g.L� (106.92 g.g�) after 144 h at 25C and 130 rpm in yeast malt (YM) medium containing 45.95 g.L� glucose 1 g.L� malt extract 0.7 g.L� yeast extract and 0.4 g.L� peptone with an initial pH value of 6. This result showed the potential of this yeast as a viable source of biopigments.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(04)%202019/16%20-...

Abstract

Several artificial colouring agents are used as food additives to improve the foods visual appearance. A recent increase in the use of natural colouring with bioactive properties (antioxidants) as a substitute for additives used in the food industry has led to the search for novel sources to produce such substances with functional colouring. The present work was aimed to isolate and select yeasts from the Cerrado (Savannah) biome (Central region Brazil) to produce carotenoids. Sixty-nine of the 470 colonies selected after screening presented colours from yellow to red. These yeasts were grouped into three colours: yellow pink and orange. Yeast belonging to the pink group identified as Rhodotorula toruloides was chosen for improvement of the factors (physical and nutritional) involved in submerged cultivation. Carotenoid bioproduction was improved by using an experimental design which evaluated the characteristics of the physical processes (agitation and temperature) followed by 2�� factorial experimental design to select the relevant factors for the culture medium. Following statistical analysis a complete second-order experimental design was employed to optimise the composition of the culture medium. The maximum carotenoid production obtained was 1 333.11 g.L� (106.92 g.g�) after 144 h at 25C and 130 rpm in yeast malt (YM) medium containing 45.95 g.L� glucose 1 g.L� malt extract 0.7 g.L� yeast extract and 0.4 g.L� peptone with an initial pH value of 6. This result showed the potential of this yeast as a viable source of biopigments.

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Item Type:	Article
AGROVOC Term:	Carotenoids
AGROVOC Term:	Biological production
AGROVOC Term:	Isolation
AGROVOC Term:	Rhodotorula
AGROVOC Term:	Yeasts
AGROVOC Term:	Pigments
AGROVOC Term:	Food industry
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8511

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