Low-cost production of chitosanolytic enzymes from Lentzea sp. strain OUR-I1 for the production of antimicrobial substances against food-borne pathogens

Yano S., . and Suyotha W., . and Konno H., . and Cheirsilp B., . and Huynh N. T., . and Wakayama M., . Low-cost production of chitosanolytic enzymes from Lentzea sp. strain OUR-I1 for the production of antimicrobial substances against food-borne pathogens. pp. 1293-1304. ISSN 2231-7546

Bioactive compounds derived from chitosan are exploited in various applications and enzymatic hydrolysis of chitosan substrate using chitosanases is a promising approach to prepare these compounds. However low yield and high cost of enzyme production still limit its use. The use of agricultural residues is an alternative to reduce the enzyme production costs. The present work aimed to use shrimp shell as a cheap substrate for the production of chitosanase and to apply the obtained enzyme in producing antimicrobial compounds. The isolated Lentzea sp. strain OUR-I1 was able to use shrimp shell waste as a nutrient source and exhibited high chitosanase activity. Although shrimp shell powder (1.5 w/v) could be used as the main carbon source in producing chitosanase the addition of chitosan powder (0.05 w/v) as an inducer substantially increased chitosanase activity. Other factors involved in the production of chitosanase were also optimised. Under these optimised conditions the obtained amount of chitosanase increased by about 17-fold from 0.14 0.006 to 2.4 0.038 U/mL. Additionally the partially purified chitosanase could effectively hydrolyse insoluble forms of chitosan into a hydrolysate mixture which in turn exhibited antimicrobial activities against a broad spectrum of food-pathogenic bacteria. The present work proposes a cost-efficient and environmentally friendly approach to produce chitosanase by a newly isolated bacterial strain and demonstrates high potential of the obtained enzyme in the preparation of antimicrobial compounds.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(04)%202019/20%20-...

Abstract

Bioactive compounds derived from chitosan are exploited in various applications and enzymatic hydrolysis of chitosan substrate using chitosanases is a promising approach to prepare these compounds. However low yield and high cost of enzyme production still limit its use. The use of agricultural residues is an alternative to reduce the enzyme production costs. The present work aimed to use shrimp shell as a cheap substrate for the production of chitosanase and to apply the obtained enzyme in producing antimicrobial compounds. The isolated Lentzea sp. strain OUR-I1 was able to use shrimp shell waste as a nutrient source and exhibited high chitosanase activity. Although shrimp shell powder (1.5 w/v) could be used as the main carbon source in producing chitosanase the addition of chitosan powder (0.05 w/v) as an inducer substantially increased chitosanase activity. Other factors involved in the production of chitosanase were also optimised. Under these optimised conditions the obtained amount of chitosanase increased by about 17-fold from 0.14 0.006 to 2.4 0.038 U/mL. Additionally the partially purified chitosanase could effectively hydrolyse insoluble forms of chitosan into a hydrolysate mixture which in turn exhibited antimicrobial activities against a broad spectrum of food-pathogenic bacteria. The present work proposes a cost-efficient and environmentally friendly approach to produce chitosanase by a newly isolated bacterial strain and demonstrates high potential of the obtained enzyme in the preparation of antimicrobial compounds.

[error in script]

Item Type:	Article
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Enzymes
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Microorganisms
AGROVOC Term:	Agricultural wastes
AGROVOC Term:	Chitosan
AGROVOC Term:	Penaeus monodon
AGROVOC Term:	Prawns and shrimps
AGROVOC Term:	Enzymatic activity
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8517

Actions (login required)

View Item