Evaluation of the antibacterial and sporicidal activity of the essential oils of Copaifera multijuga and Thymus vulgaris against Alicyclobacillus acidoterrestris

Ruiz S. P., . and Machinski Junior M., . and Graton Mikcha J. M., . and Pascoli I. C., . and Silva A. A., . and Santos I. F., . and Nakamura C. V., . and Ueda Nakamura T., . and Anjos M. M., . and Abreu Filho B. A., . Evaluation of the antibacterial and sporicidal activity of the essential oils of Copaifera multijuga and Thymus vulgaris against Alicyclobacillus acidoterrestris. pp. 1327-1337. ISSN 2231-7546

The present work aimed to evaluate the antibacterial activity of the essential oils (EOs) of Copaifera multijuga and Thymus vulgaris against Alicyclobacillus acidoterrestris. The minimum inhibitory concentration (MIC) of the C. multijuga and T. vulgaris EOs were 300 and 500 g/mL respectively and the minimum bactericidal concentration (MBC) of both EOs was 1 000 g/mL. The best minimum sporicidal concentration (MSC) of C. multijuga was reached at a concentration of 500 g/mL with a reduction of 3.07 log CFU/mL. For the EO of T. vulgaris better results were observed at concentrations higher than 500 g/mL and a reduction of 2.05 log CFU/mL was achieved at 1 000 g/mL. The checkerboard method showed that the combination of EOs and nisin had an additive interaction. The bactericidal activity was confirmed by the death curve. According to the selectivity index the treatment was less selective for A. acidoterrestris than for Vero cells. The flow cytometry results indicated that the vegetative forms of A. acidoterrestris had a higher incidence with alterations in cell membrane integrity as compared to their spores. When EOs were added to reconstituted orange juice antibacterial treatment was highly effective and when EOs were combined with nisin there was a complete reduction of bacterial load. Thus it is evident that the EOs of C. multijuga and T. vulgaris have potential for use as antibacterial agents against A. acidoterrestris.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(04)%202019/23%20-...

Abstract

The present work aimed to evaluate the antibacterial activity of the essential oils (EOs) of Copaifera multijuga and Thymus vulgaris against Alicyclobacillus acidoterrestris. The minimum inhibitory concentration (MIC) of the C. multijuga and T. vulgaris EOs were 300 and 500 g/mL respectively and the minimum bactericidal concentration (MBC) of both EOs was 1 000 g/mL. The best minimum sporicidal concentration (MSC) of C. multijuga was reached at a concentration of 500 g/mL with a reduction of 3.07 log CFU/mL. For the EO of T. vulgaris better results were observed at concentrations higher than 500 g/mL and a reduction of 2.05 log CFU/mL was achieved at 1 000 g/mL. The checkerboard method showed that the combination of EOs and nisin had an additive interaction. The bactericidal activity was confirmed by the death curve. According to the selectivity index the treatment was less selective for A. acidoterrestris than for Vero cells. The flow cytometry results indicated that the vegetative forms of A. acidoterrestris had a higher incidence with alterations in cell membrane integrity as compared to their spores. When EOs were added to reconstituted orange juice antibacterial treatment was highly effective and when EOs were combined with nisin there was a complete reduction of bacterial load. Thus it is evident that the EOs of C. multijuga and T. vulgaris have potential for use as antibacterial agents against A. acidoterrestris.

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Item Type:	Article
AGROVOC Term:	Essential oils
AGROVOC Term:	Essences (essential oils)
AGROVOC Term:	Copaifera
AGROVOC Term:	Thymus (genus)
AGROVOC Term:	Evaluation
AGROVOC Term:	Antimicrobial resistance
AGROVOC Term:	Gram positive bacteria
AGROVOC Term:	Bacterial spores
AGROVOC Term:	Orange juice
AGROVOC Term:	Inhibition
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8533

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