Citation
Zeenathul N.A., . and Mohd Azmi M. L., . and Loh H. S., . and Sheikh Omar A. R., . and Zamri Saad M., . Molecular characterisation and identification of the major immediate-early (MIE) genes of rat cytomegalovirus (RCMV). pp. 21-32. ISSN 0128-2506
Abstract
The paper first describes the characterisation and identification of two transcripts of major immediate-early (MIE) genes of rat cytomegalovirus (RCMV) strain ALL-03 using molecular approach. In the absence of any known sequences in the strain ALL-03 IE genes the first recognised viral genes expressed during infection were the most important genetic source for the exploration of the viral genome. The steps taken were the construction of cDNA libraries from rat embryonic fibroblast (REF) infected with RCMV strain All-03 under IE� condition by using several techniques such as RT-PCR PCR cloning sequencing and bioinformatic. Two transcripts were confirmed to be of RCMV strain ALL-03 origin by specific PCR and hybridisation analyses. Based on the bioinformatics analyses it is most likely that these transcripts are exon 4(IE1) and exon 5 (IE2) genes. The considerable divergence in the nucleotide sequence of the two IE transcripts from those of MIE regions of both Maastrict and English strains further supports the claim that the All-03 is a distinct RCMV starin indeed.
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Abstract
The paper first describes the characterisation and identification of two transcripts of major immediate-early (MIE) genes of rat cytomegalovirus (RCMV) strain ALL-03 using molecular approach. In the absence of any known sequences in the strain ALL-03 IE genes the first recognised viral genes expressed during infection were the most important genetic source for the exploration of the viral genome. The steps taken were the construction of cDNA libraries from rat embryonic fibroblast (REF) infected with RCMV strain All-03 under IE� condition by using several techniques such as RT-PCR PCR cloning sequencing and bioinformatic. Two transcripts were confirmed to be of RCMV strain ALL-03 origin by specific PCR and hybridisation analyses. Based on the bioinformatics analyses it is most likely that these transcripts are exon 4(IE1) and exon 5 (IE2) genes. The considerable divergence in the nucleotide sequence of the two IE transcripts from those of MIE regions of both Maastrict and English strains further supports the claim that the All-03 is a distinct RCMV starin indeed.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Cytomegalovirus |
| AGROVOC Term: | Molecular genetics |
| AGROVOC Term: | Identification |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Gene sequence |
| AGROVOC Term: | Nucleotide sequence |
| AGROVOC Term: | Genetic divergence |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8606 |
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