Isolation of pesticide degrading bacteria from paddy fields and evaluation of its bioremediation potential efficiency

G. Manigandan, . and A. Raja, . and Vajiha Banu H., . and Gajalakshmi P., . Isolation of pesticide degrading bacteria from paddy fields and evaluation of its bioremediation potential efficiency. pp. 173-182. ISSN 1394-7990

Pesticides are chemicals that are widely used in the agricultural sector to control pests in the environment. Lambda cyhalothrin is an insecticide that belongs to a group of pyrethroids. As lambda cyhalothrin is persistent in the soil there is an urgent need to take remedial measures to control environmental pollution. In this study the ability of bacteria to degrade lambda cyhalothrin from a paddy field was evaluated. Tolerance of bacterial isolates was tested at different concentrations. Among the ten different genera isolate Pseudomonas sp designated as GMMC1 was found to tolerate pesticides up to 500 ppm and was selected for further degradation studies. GMMC1 lambda-cyhalothrin degrading bacterium identified by Sequence BLAST analysis was isolated from the paddy crop soil and found to be the dominant bacteria which tolerates pesticide. Results of a phylogenetic analysis of GMMC1 found it to be closely related to Pseudomonas fluorescens. Pseudomonas fluorescens (GMMC1) isolate as appears to be the best short term choice for bioremediation of pesticide-contaminated agricultural fields.

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Abstract

Pesticides are chemicals that are widely used in the agricultural sector to control pests in the environment. Lambda cyhalothrin is an insecticide that belongs to a group of pyrethroids. As lambda cyhalothrin is persistent in the soil there is an urgent need to take remedial measures to control environmental pollution. In this study the ability of bacteria to degrade lambda cyhalothrin from a paddy field was evaluated. Tolerance of bacterial isolates was tested at different concentrations. Among the ten different genera isolate Pseudomonas sp designated as GMMC1 was found to tolerate pesticides up to 500 ppm and was selected for further degradation studies. GMMC1 lambda-cyhalothrin degrading bacterium identified by Sequence BLAST analysis was isolated from the paddy crop soil and found to be the dominant bacteria which tolerates pesticide. Results of a phylogenetic analysis of GMMC1 found it to be closely related to Pseudomonas fluorescens. Pseudomonas fluorescens (GMMC1) isolate as appears to be the best short term choice for bioremediation of pesticide-contaminated agricultural fields.

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Item Type:	Article
AGROVOC Term:	Bacterial pesticides
AGROVOC Term:	Paddy soil
AGROVOC Term:	Bioremediation
AGROVOC Term:	Lambda-cyhalothrin
AGROVOC Term:	Pseudomonas fluorescens
AGROVOC Term:	Biodegradation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8653

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