Development of indirect and double antibody sandwich enzyme-linked immunosorbent assay for infectious bursal disease


Citation

Phong S. F., . and Hair-Bejo M., . and A. R. Omar, . and Aini I., . Development of indirect and double antibody sandwich enzyme-linked immunosorbent assay for infectious bursal disease. pp. 19-24. ISSN 0128-2506

Abstract

Indirect and double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) was successfully developed using the P97/302 field local isolate of very virulent (vv) infectious bursal disease virus (IBDV) to detect both the antibody and antigen against IBDV. The regression line equation of the indirect ELISA was Log‚�‚ Titre -1.1495(1/SP) 4.5349 with R0.975 and DAS ELISA was Log‚�‚ Titre2.6527Log‚�‚(SP)3.0853 with R0.93 which was calculated from the standard curves. Serum samples were then obtained from the commercial and specific-pathogen-free (SPF) chickens for commercial IDEXX developed indirect and DAS ELISA evaluations and the results showed significant correlation (p0.01) between IDEXX and indirect with R0.88 and between IDEXX and DAS with R0.93. The developed DAS ELISA for antigen detection is also highly specific. It can detect various IBDV field isolates from the bursa tissue and various IBDV field isolates from the bursa tissue and various IBDV vaccine strains.


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Abstract

Indirect and double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) was successfully developed using the P97/302 field local isolate of very virulent (vv) infectious bursal disease virus (IBDV) to detect both the antibody and antigen against IBDV. The regression line equation of the indirect ELISA was Log‚�‚ Titre -1.1495(1/SP) 4.5349 with R0.975 and DAS ELISA was Log‚�‚ Titre2.6527Log‚�‚(SP)3.0853 with R0.93 which was calculated from the standard curves. Serum samples were then obtained from the commercial and specific-pathogen-free (SPF) chickens for commercial IDEXX developed indirect and DAS ELISA evaluations and the results showed significant correlation (p0.01) between IDEXX and indirect with R0.88 and between IDEXX and DAS with R0.93. The developed DAS ELISA for antigen detection is also highly specific. It can detect various IBDV field isolates from the bursa tissue and various IBDV field isolates from the bursa tissue and various IBDV vaccine strains.

Additional Metadata

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Item Type: Article
AGROVOC Term: Infectious bursal disease virus
AGROVOC Term: Poultry
AGROVOC Term: Antibody formation
AGROVOC Term: Indirect ELISA
AGROVOC Term: Antibodies
AGROVOC Term: Antigens
AGROVOC Term: Viral antigens
AGROVOC Term: Vaccines
AGROVOC Term: Viral diseases
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8658

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