Citation
Nurul Farah Diyana Ahmad Tahir, . and Mohd Firdaus Ariff Abdul Razak, . and Salmah Yaakop, . and Maldonado Jesús E, . and Kayal Vizi Karuppannan, . and Badrul Munir Md Zain, . and Norsyamimi Rosli, . Influence of Asian elephant dung decay on DNA recovery. pp. 175-181. ISSN 1823-8556
Abstract
Evaluation on Asian elephant dung decay rate affecting the yield of DNA was carried out in National Elephant Conservation Center (NECC) Kuala Gandah Pahang Peninsular Malaysia. The attempt is intended to optimise the current field DNA sampling method. Two distinguished environmental sites of dung piles were sampled; direct sunlight exposures and under shades of voluminous tree canopies. Dung piles of Sanum a cow; and Rajah a bull were being tested. Results showed that the environmental factors of sample sources have not affected the purity of DNA. Nonetheless the DNA quantity varies between both study sites samples. The average of DNA concentration amongst the shady area samples was 7.6 ng/L whereby in sunny area the concentration was lower at 6.0 ng/L. It is showed that better quantity of DNA are related to dung decay rates where faecal samples with direct sunlight will degrade faster compared to shady areas covered by canopies. This outcome contributes a supporting data for non-invasive field DNA sampling on Asian elephants.
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Abstract
Evaluation on Asian elephant dung decay rate affecting the yield of DNA was carried out in National Elephant Conservation Center (NECC) Kuala Gandah Pahang Peninsular Malaysia. The attempt is intended to optimise the current field DNA sampling method. Two distinguished environmental sites of dung piles were sampled; direct sunlight exposures and under shades of voluminous tree canopies. Dung piles of Sanum a cow; and Rajah a bull were being tested. Results showed that the environmental factors of sample sources have not affected the purity of DNA. Nonetheless the DNA quantity varies between both study sites samples. The average of DNA concentration amongst the shady area samples was 7.6 ng/L whereby in sunny area the concentration was lower at 6.0 ng/L. It is showed that better quantity of DNA are related to dung decay rates where faecal samples with direct sunlight will degrade faster compared to shady areas covered by canopies. This outcome contributes a supporting data for non-invasive field DNA sampling on Asian elephants.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Elephants |
| AGROVOC Term: | Dung |
| AGROVOC Term: | Decay |
| AGROVOC Term: | Evaluation |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Grasslands |
| AGROVOC Term: | Nucleic acids |
| AGROVOC Term: | Gel electrophoresis |
| AGROVOC Term: | Purity |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8697 |
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