Citation
Wong C. W., . and Ding K. Y., . and Anggraeni V. S., . Aqueous two-phase separation (ATPS) of polyphenol oxidase from lotus root (Nelumbo nucifera) and its characterisation. pp. 1699-1706. ISSN 0116-6514
Abstract
Polyphenol oxidase (PPO) was extracted from lotus (Nelumbo nucifera) root and partially purified up to 11.51 with a 43.6 yield using aqueous two-phase separation system. The optimum pH was at 8.0 for both substrates; 4-methylcatechol and pyrogallol. PPO activities were maximally achieved at 50C for pyrogallol and 20C for 4-methylcatechol. 84 and 71 loss of relative PPO activities were obtained for pyrogallol and 4-methylcatechol respectively following heat inactivation at 90C for 40 min. Inactivation rate constant (k) values ranged between 0.88 10- min- to 2.97 10- min- for pyrogallol and 0.69 10- min- to 2.44 10- min- for 4-methylcatechol. The activation energy (Ea) were 75.32 kJ mol- and 73.14 kJ mol- for pyrogallol and 4-methylcatechol respectively. PPO activity was strongly inhibited ( 79) by ascorbic acid. SDS exhibited the greatest efficiency in activating lotus root PPO.
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Abstract
Polyphenol oxidase (PPO) was extracted from lotus (Nelumbo nucifera) root and partially purified up to 11.51 with a 43.6 yield using aqueous two-phase separation system. The optimum pH was at 8.0 for both substrates; 4-methylcatechol and pyrogallol. PPO activities were maximally achieved at 50C for pyrogallol and 20C for 4-methylcatechol. 84 and 71 loss of relative PPO activities were obtained for pyrogallol and 4-methylcatechol respectively following heat inactivation at 90C for 40 min. Inactivation rate constant (k) values ranged between 0.88 10- min- to 2.97 10- min- for pyrogallol and 0.69 10- min- to 2.44 10- min- for 4-methylcatechol. The activation energy (Ea) were 75.32 kJ mol- and 73.14 kJ mol- for pyrogallol and 4-methylcatechol respectively. PPO activity was strongly inhibited ( 79) by ascorbic acid. SDS exhibited the greatest efficiency in activating lotus root PPO.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Polyphenol oxidase |
| AGROVOC Term: | Lotus |
| AGROVOC Term: | Nelumbo nucifera |
| AGROVOC Term: | Ascorbic acid |
| AGROVOC Term: | Separation |
| AGROVOC Term: | Inhibition |
| AGROVOC Term: | Enzymatic browning |
| AGROVOC Term: | Fruits |
| AGROVOC Term: | Vegetables |
| AGROVOC Term: | Food quality |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8751 |
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