Physicochemical analysis and antioxidant activity of honey from three Malaysian stingless bees species


Citation

Muhammad Muslim Mohd Rodi, . and Khamsah Suryati Mohd, . and Abdul Jamil Zakaria, . and Noorazlin Mohd Nasir, . and Nurhamizah Ibrahim, . Physicochemical analysis and antioxidant activity of honey from three Malaysian stingless bees species. pp. 1-11. ISSN 2180-1983

Abstract

This study investigates physicochemical and antioxidant activity of raw and honey extract from three different species of Malaysian stingless bees. The methanol extract of commonly found Heterotrigona itama (EHI) Tetratgonulalaeviceps (ETL) and Geniotrigona thoracica (EGT) was tested for their total flavonoid total phenolic moisture ash fat protein and carbohydrate. Antioxidant activity through DPPH radical scavenging activity was also determined. Nutritional analysis of raw honey showed a comparable result. Analysis of total flavonoid and total phenolic for honey extract showed a significant difference between three species. In contrast raw honeys did not show any significant differences between species. The antioxidant testing showed that the three honeys do not possess good antioxidant activity with inhibition lower than 50. ETL showed the highest inhibition with IC50 of 37.8 followed by EHI with 24.3 and EGT with 9.8 of inhibition respectively. Thin layer chromatography analysis revealed the content of phenolic and flavonoids in all tested honey. The three extracts displayed a characteristic profile and vary from each other. This study revealed that bee species is important in determination of honeys quality.


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Abstract

This study investigates physicochemical and antioxidant activity of raw and honey extract from three different species of Malaysian stingless bees. The methanol extract of commonly found Heterotrigona itama (EHI) Tetratgonulalaeviceps (ETL) and Geniotrigona thoracica (EGT) was tested for their total flavonoid total phenolic moisture ash fat protein and carbohydrate. Antioxidant activity through DPPH radical scavenging activity was also determined. Nutritional analysis of raw honey showed a comparable result. Analysis of total flavonoid and total phenolic for honey extract showed a significant difference between three species. In contrast raw honeys did not show any significant differences between species. The antioxidant testing showed that the three honeys do not possess good antioxidant activity with inhibition lower than 50. ETL showed the highest inhibition with IC50 of 37.8 followed by EHI with 24.3 and EGT with 9.8 of inhibition respectively. Thin layer chromatography analysis revealed the content of phenolic and flavonoids in all tested honey. The three extracts displayed a characteristic profile and vary from each other. This study revealed that bee species is important in determination of honeys quality.

Additional Metadata

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Item Type: Article
AGROVOC Term: Honey
AGROVOC Term: Honey bees
AGROVOC Term: Bees
AGROVOC Term: Apidae
AGROVOC Term: Physicochemical properties
AGROVOC Term: Phenolic content
AGROVOC Term: Flavonoids
AGROVOC Term: Chromatography
AGROVOC Term: Nutritional value
AGROVOC Term: Antioxidants
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8772

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