Citation
Nor Adilah Azmi, . and Tajul Afif Abdullah, . and Nor Hasima Mahmod, . and Mohammad Hailmi Sajili, . and Khairil Mahmud, . and Salmah Mohamed, . and Khandaker Moneruzzaman, . Sterilization of Anubias nana and Rotala macrandra for In Vitro culture. pp. 13-21. ISSN 0116-6514
Abstract
This study was conducted to establish a sterilization protocol for Anubias nana and Rotala macrandra. The plants from its originated area are highly exposed to microbial contamination such as bacteria and fungi. Those contaminants can be eliminated by using suitable sterilizing agents. The sterilization is a vital step in preparing a healthy and viable explant in tissue culture. In this study three different sterilizing agents which are fungicide silver nitrate (AgNO3) and mercuric chloride (HgC1‚‚) were used. The percentages of survived and contaminated explants were observed after one week of culture. The results showed that 100 of Anubias nana explant survived when the explants were rapidly rinsed with 0.1 (HgC1‚‚). The explant of Rotala macrandra resulted in 56 of survival when it was rapid rinsed with 0.05 HgC1‚‚. However treatment 0.05 HgC1‚‚ for Rotala macrandra also produced 33 of fungal contamination.
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Abstract
This study was conducted to establish a sterilization protocol for Anubias nana and Rotala macrandra. The plants from its originated area are highly exposed to microbial contamination such as bacteria and fungi. Those contaminants can be eliminated by using suitable sterilizing agents. The sterilization is a vital step in preparing a healthy and viable explant in tissue culture. In this study three different sterilizing agents which are fungicide silver nitrate (AgNO3) and mercuric chloride (HgC1‚‚) were used. The percentages of survived and contaminated explants were observed after one week of culture. The results showed that 100 of Anubias nana explant survived when the explants were rapidly rinsed with 0.1 (HgC1‚‚). The explant of Rotala macrandra resulted in 56 of survival when it was rapid rinsed with 0.05 HgC1‚‚. However treatment 0.05 HgC1‚‚ for Rotala macrandra also produced 33 of fungal contamination.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Araceae |
| AGROVOC Term: | Lythraceae |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Sterilization |
| AGROVOC Term: | Fungicides |
| AGROVOC Term: | Mercuric chloride |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Survival |
| AGROVOC Term: | Fungal contamination |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8773 |
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