Identification and characterization of the causal agent of infected iceberg lettuce (Lactuca Sativa L.) in Perak Malaysia

Saiful Iskandar Khalit, . and Noor Afiza Badaluddin, . and Mohammad Hailmi Sajili, . and Sufi Diyanah Mohd Nazeri, . and Nur Aisyah Zin, . Identification and characterization of the causal agent of infected iceberg lettuce (Lactuca Sativa L.) in Perak Malaysia. pp. 59-67. ISSN 2180-1983

Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops which is causing negative effects on environment and humans health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sativaL.) in Perak Malaysia. The infected iceberg lettuces were sampling from Perak area and the causal agent was isolated by direct plating technique on a nutrient medium. The isolated bacteria were identified by molecular method and biochemical test. Molecular identification was carried out using 16S rRNA sequence and then several tests such as amylase catalase oxidase and others were done for identification of bacterial species based on their biochemical activities. The obtained results showed that Serratia marcescenes and Stenotrophomonas sp. were identified as the causal agents. The pathogenicity has been confirmed by the establishment of Kochs Postulate which showed 100 disease infection on them. The environmental effects also have been tested on the bacteria to study the ability of bacteria to grow in different environmental conditions based on different in pH salinity and temperature. All the bacterial sample showed that they able to growth on the pH between 4.0 to 9.0 at the temperature 10 C to 45 C and at the addition salinity to the medium between 2.5 5.0 and 7.5 .

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops which is causing negative effects on environment and humans health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sativaL.) in Perak Malaysia. The infected iceberg lettuces were sampling from Perak area and the causal agent was isolated by direct plating technique on a nutrient medium. The isolated bacteria were identified by molecular method and biochemical test. Molecular identification was carried out using 16S rRNA sequence and then several tests such as amylase catalase oxidase and others were done for identification of bacterial species based on their biochemical activities. The obtained results showed that Serratia marcescenes and Stenotrophomonas sp. were identified as the causal agents. The pathogenicity has been confirmed by the establishment of Kochs Postulate which showed 100 disease infection on them. The environmental effects also have been tested on the bacteria to study the ability of bacteria to grow in different environmental conditions based on different in pH salinity and temperature. All the bacterial sample showed that they able to growth on the pH between 4.0 to 9.0 at the temperature 10 C to 45 C and at the addition salinity to the medium between 2.5 5.0 and 7.5 .

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Item Type:	Article
AGROVOC Term:	Lettuces
AGROVOC Term:	Lactuca sativa
AGROVOC Term:	Vegetable crops
AGROVOC Term:	Molecular biology
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Identification
AGROVOC Term:	Bacteria
AGROVOC Term:	Serratia
AGROVOC Term:	Infection
AGROVOC Term:	Salads
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8783

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