In Vitro micropropagation of aquarium plants pearl grass Hemianthus micranthemoides (Nuttall) and micro sword grass Lilaeopsis brasiliensis (Glaziou) Affolter (Apiaceae)

Norhanizan Sahidin, . and Anis Athirah Kharuddin, . and Nor Hasima Mahmud, . and Tajul Afif Abdullah, . and Nguang Siew Inga, . and Ha Hou Chew, . and Rokiah Zainuddin, . In Vitro micropropagation of aquarium plants pearl grass Hemianthus micranthemoides (Nuttall) and micro sword grass Lilaeopsis brasiliensis (Glaziou) Affolter (Apiaceae). pp. 88-93. ISSN 2180-1983

Hemianthus micranthemoides and Lileaopsis brasiliensis are nowadays sold in the form of tissue culture production by aquaria and ornamental trade in Malaysia. However there is no to limited report about in vitro micropropagation of both aquarium plants. In this study an experimental procedure for micropropagation of both plants was documented. The propagation was done in Murashige and Skoog (MS) medium with eight combinations of different concentration of 6-Benzylaminopurine with Naphteneacetic acid (BAP-NAA) and 1-phenyl-3-1 2 3-thiadizol-5-yl urea with Naphteneacetic acid (TDZ-NAA) following the rooting technique for 42 days. All treatments for 1 L MS medium were regulated to pH 5.7 to 5.8 and added with sucrose (30 g/L) and phytagel (2.5 g/L). All combination treatments had induced rooting. There was a significance different in the shoot regeneration of H. micranthemoidesin all treatment (95 confidence level F 13.907 n 240 p 0.000) on the contrary there was no different were observed in shoot regeneration of L. brasiliensis treatment. Overall all treatment of TDZ-NAA were significantly higher in the shoot regeneration than those in BAP-NAA treatment. Particularly the T7(TDZ-NAA 0.075: 0.1 mg/L) given highest result in diameter (1.6260.213 cm) and area (1.7240.308 cm2) of shoot clumps in H. micranthemoides and formation of shoots (51.54.95) nodes (20.02.83) and roots (45.513.4) in L. brasiliensis. Hardening of in vitro cultured plantlets was done in aquatic laboratory. All of the plants survived and transferred into aquarium. This study has provided beneficial information in further micropropagation protocol for the mass production of both aquarium plants

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Hemianthus micranthemoides and Lileaopsis brasiliensis are nowadays sold in the form of tissue culture production by aquaria and ornamental trade in Malaysia. However there is no to limited report about in vitro micropropagation of both aquarium plants. In this study an experimental procedure for micropropagation of both plants was documented. The propagation was done in Murashige and Skoog (MS) medium with eight combinations of different concentration of 6-Benzylaminopurine with Naphteneacetic acid (BAP-NAA) and 1-phenyl-3-1 2 3-thiadizol-5-yl urea with Naphteneacetic acid (TDZ-NAA) following the rooting technique for 42 days. All treatments for 1 L MS medium were regulated to pH 5.7 to 5.8 and added with sucrose (30 g/L) and phytagel (2.5 g/L). All combination treatments had induced rooting. There was a significance different in the shoot regeneration of H. micranthemoidesin all treatment (95 confidence level F 13.907 n 240 p 0.000) on the contrary there was no different were observed in shoot regeneration of L. brasiliensis treatment. Overall all treatment of TDZ-NAA were significantly higher in the shoot regeneration than those in BAP-NAA treatment. Particularly the T7(TDZ-NAA 0.075: 0.1 mg/L) given highest result in diameter (1.6260.213 cm) and area (1.7240.308 cm2) of shoot clumps in H. micranthemoides and formation of shoots (51.54.95) nodes (20.02.83) and roots (45.513.4) in L. brasiliensis. Hardening of in vitro cultured plantlets was done in aquatic laboratory. All of the plants survived and transferred into aquarium. This study has provided beneficial information in further micropropagation protocol for the mass production of both aquarium plants

[error in script]

Item Type:	Article
AGROVOC Term:	Aquatic plants
AGROVOC Term:	Aquatic ornamental plants
AGROVOC Term:	Scrophulariaceae
AGROVOC Term:	Apiaceae
AGROVOC Term:	Aquaria
AGROVOC Term:	Micropropagation
AGROVOC Term:	In vitro culture
AGROVOC Term:	In vitro regeneration
AGROVOC Term:	Culture media
AGROVOC Term:	Shoots
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8786

Actions (login required)

View Item