SureSawitTM true-to-type - a high throughput universal single nucleotide polymorphism panel for dna fingerprinting purity testing and origin verification in oil palm

Ooi Leslie Cheng Li, . and Sander Nathan, . and Lakey Nathan, . and Garner Peggy A, . and Marjuni Marhalil, . and Bacher Blaire, . and Low Leslie Eng-Ti, . and Jiang Nan, . and Meilina Ong Abdullah, . and Smith Steve, . and Nookiah Rajanaidu, . and Ordway Jared, . and Ong Pei Wen, . and Leinenger Michael T., . and Zulkifli Yaakub, . and Mohamad Arif Abd Manaf, . and Chan Pek-Lan, . and Rajinder Singh, . and Ravigadevi Sambanthamurthi, . SureSawitTM true-to-type - a high throughput universal single nucleotide polymorphism panel for dna fingerprinting purity testing and origin verification in oil palm. pp. 561-571. ISSN 1511-2780

A high performing True-to-Type single nucleotide polymorphism assay consisting of a minimal set of single nucleotide polymorphism markers was developed for oil palm. The single nucleotide polymorphism panel was developed from a range of diverse materials consisting of germplasm and advanced breeding lines. Analyses demonstrated that the single nucleotide polymorphism panel is effective at uniquely finger printing and discriminating individual palms from unrelated pedigrees. In addition the single nucleotide polymorphism panel is a very useful tool in breeding programmes as it can effectively discriminate between individual progeny in half-sibling full sibling and selfed crosses. Furthermore illegitimate palms present in half-sibling full sibling and selfed crosses are easily identified. The single nucleotide polymorphism panel is an excellent quality control tool for use in tissue culture as clonal materials could be differentiated based on their lineage. The fact that ramets of different clonal lines were easily distinguished indicates that it will also be an excellent tool to identify potential culture mix-ups that occur either in the laboratory nursery or during field planting.

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Abstract

A high performing True-to-Type single nucleotide polymorphism assay consisting of a minimal set of single nucleotide polymorphism markers was developed for oil palm. The single nucleotide polymorphism panel was developed from a range of diverse materials consisting of germplasm and advanced breeding lines. Analyses demonstrated that the single nucleotide polymorphism panel is effective at uniquely finger printing and discriminating individual palms from unrelated pedigrees. In addition the single nucleotide polymorphism panel is a very useful tool in breeding programmes as it can effectively discriminate between individual progeny in half-sibling full sibling and selfed crosses. Furthermore illegitimate palms present in half-sibling full sibling and selfed crosses are easily identified. The single nucleotide polymorphism panel is an excellent quality control tool for use in tissue culture as clonal materials could be differentiated based on their lineage. The fact that ramets of different clonal lines were easily distinguished indicates that it will also be an excellent tool to identify potential culture mix-ups that occur either in the laboratory nursery or during field planting.

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Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Progeny testing
AGROVOC Term:	Genetic markers
AGROVOC Term:	DNA fingerprinting
AGROVOC Term:	Genetic variability
AGROVOC Term:	Plant breeding
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8796

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