Molecular investigation of feline coronavirus (FCOV) in local pet cats

K. Mummoorthy, . and Yasmin A. R., . and A. R. Omar, . and P. Anand, . and Hoong L. W., . and K. Kumar, . and S. S. Arshad, . Molecular investigation of feline coronavirus (FCOV) in local pet cats. pp. 13-18. ISSN 0128-2506

Feline coronavirus (FCoV) infection is a very common in cat population. FCoV is further classified into two biotypes namely feline enteric coronavirus (FECV) and mutated feline infectious peritonitis virus (FIPV) in which FIPV causes a fatal immune complex disease by changing the tropism from enterocytes to monocytes. Previous studies on molecular detection of FCoV in cats were carried out in catteries but limited study investigate the presence of FCoV antigen in local pet cats. By considering this fact this study aims to detect FCoV antigen via RT-PCR assay in local pet cats and to compare the similarity of the identified FCoV strain with previous related virus by phylogenetic analysis. By using convenience sampling rectal swabs and buffy coat were collected from 16 clinically ill pet cats and 5 healthy pet cats. Viral RNA was extracted and subjected to one-step RT-PCR targeting polymerase gene. Only one out of 21 fecal samples was positive for FCoV and none from buffy coat samples. Phylogenetic analysis revealed that the identified positive sample was highly homologous up to 95 to FCoV strain from Netherlands and South Korea on partial sequence of polymerase gene. In conclusion this study detected FCoV antigen in local pet cats from fecal samples while negative detection from fecal and buffy coat samples could not completely rule out the possibilities of FCoV infection due to the complexity of the virus diagnosis that require multiple series of analysis.

Full text available from: Official URL: https://www.mavma.org.my/2019-volume-31-issue-no-2...

Abstract

Feline coronavirus (FCoV) infection is a very common in cat population. FCoV is further classified into two biotypes namely feline enteric coronavirus (FECV) and mutated feline infectious peritonitis virus (FIPV) in which FIPV causes a fatal immune complex disease by changing the tropism from enterocytes to monocytes. Previous studies on molecular detection of FCoV in cats were carried out in catteries but limited study investigate the presence of FCoV antigen in local pet cats. By considering this fact this study aims to detect FCoV antigen via RT-PCR assay in local pet cats and to compare the similarity of the identified FCoV strain with previous related virus by phylogenetic analysis. By using convenience sampling rectal swabs and buffy coat were collected from 16 clinically ill pet cats and 5 healthy pet cats. Viral RNA was extracted and subjected to one-step RT-PCR targeting polymerase gene. Only one out of 21 fecal samples was positive for FCoV and none from buffy coat samples. Phylogenetic analysis revealed that the identified positive sample was highly homologous up to 95 to FCoV strain from Netherlands and South Korea on partial sequence of polymerase gene. In conclusion this study detected FCoV antigen in local pet cats from fecal samples while negative detection from fecal and buffy coat samples could not completely rule out the possibilities of FCoV infection due to the complexity of the virus diagnosis that require multiple series of analysis.

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Item Type:	Article
AGROVOC Term:	Cats
AGROVOC Term:	Coronavirus
AGROVOC Term:	Diagnosis
AGROVOC Term:	Blood sampling
AGROVOC Term:	Faeces
AGROVOC Term:	RNA
AGROVOC Term:	Genes
AGROVOC Term:	DNA sequence
AGROVOC Term:	Nucleotide sequence
AGROVOC Term:	Reverse transcriptase
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8811

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