Citation
Shaharum S., . and Zawawi L., . and Abas Mazni O., . and Hawari H., . A comparison between heat and cold shock treatments in amplification of ZFX/ZFY gene for embryo sexing. pp. 11-15. ISSN 9128-2506
Abstract
Early in vitro fertilised (IVF)-derived blastocysts were bisected using a stainless steel micro-blade attached to a micro-manipulator. A small portion of the bisected samples was then exposed to either heat or ultra-cold shock treatment to burst the blastomere cell membrane. The treated samples were then subjected to polymerase chain reaction (PCR) amplification of the ZFX/ZFY gene using P1-5EZ and P2-3EZ primers. The sex of each sample was diagnosed based on the presence of Pst I-RFLP on the PCR amplified DNA of the ZFX/ZFY locus. Both heat and ultra-cold shock treatments gave similar amplification of the ZFX/ZFY gene.
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Official URL: https://www.mavma.org.my/1998-volume-10-issue-no-1...
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Abstract
Early in vitro fertilised (IVF)-derived blastocysts were bisected using a stainless steel micro-blade attached to a micro-manipulator. A small portion of the bisected samples was then exposed to either heat or ultra-cold shock treatment to burst the blastomere cell membrane. The treated samples were then subjected to polymerase chain reaction (PCR) amplification of the ZFX/ZFY gene using P1-5EZ and P2-3EZ primers. The sex of each sample was diagnosed based on the presence of Pst I-RFLP on the PCR amplified DNA of the ZFX/ZFY locus. Both heat and ultra-cold shock treatments gave similar amplification of the ZFX/ZFY gene.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Cattle |
| AGROVOC Term: | Sex determination |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Animal embryos |
| AGROVOC Term: | In vitro embryo production |
| AGROVOC Term: | Heat shock |
| AGROVOC Term: | Cold shock |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Sex |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8828 |
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