Vaccination of sheep against pneumonic pasteurellosis using a new spray vaccine

M. Zamri Saad, . and A. W. M. Effendy, . and M. Mohamad, . and H. Omar, . Vaccination of sheep against pneumonic pasteurellosis using a new spray vaccine. pp. 17-20. ISSN 9128-2506

A vaccination trial was carried out in an established sheep farm using a newly developed pasteurella spray vaccine. Fifty animals of age ranging from 1 to 3 years old in group 1 were vaccinated by spraying intranasally with 1mL of the spray vaccine while another 50 animals in group 2 were vaccinated subcutaneously with 3 mL of pasteurella alum precipitate vaccine. Vaccination of group 1 was repeated after 2 weeks while group 2 after 4 weeks. Fifty animals of group 3 were unvaccinated control. Serum samples were collected prior to and at two-weekly intervals for a period of 4 months and subjected to enzyme-linked immunosorbent assay (ELISA) to determine the antibody levels. The lungs of dead animals were submitted for bacteriological examinations. Prior to the trial the antibody levels in all three groups were low. Following vaccination the antibody levels increased steadily and significantly in both vaccinated groups and reached the peak level at week 8 post-vaccination before they declined to become significantly low at week 12 post-vaccination. The antibody level dropped to the original prior vaccination. Revaccination at week 14 significantly increased the antibody levels which lasted until the end of the study period at week 18. Stress due to haemonchosis at week 4 however drastically reduced the antibody levels in all groups causing 13 of the animals all of which were from the control group except one from the alum vaccinated group to succumb to pneumonic pasteurellosis. The result indicated that the herd must be re-vaccinated either with spray or alum vacine at 12 week intervals to maintain a protective herd immunity.

Full text available from: Official URL: https://www.mavma.org.my/1998-volume-10-issue-no-1...

Abstract

A vaccination trial was carried out in an established sheep farm using a newly developed pasteurella spray vaccine. Fifty animals of age ranging from 1 to 3 years old in group 1 were vaccinated by spraying intranasally with 1mL of the spray vaccine while another 50 animals in group 2 were vaccinated subcutaneously with 3 mL of pasteurella alum precipitate vaccine. Vaccination of group 1 was repeated after 2 weeks while group 2 after 4 weeks. Fifty animals of group 3 were unvaccinated control. Serum samples were collected prior to and at two-weekly intervals for a period of 4 months and subjected to enzyme-linked immunosorbent assay (ELISA) to determine the antibody levels. The lungs of dead animals were submitted for bacteriological examinations. Prior to the trial the antibody levels in all three groups were low. Following vaccination the antibody levels increased steadily and significantly in both vaccinated groups and reached the peak level at week 8 post-vaccination before they declined to become significantly low at week 12 post-vaccination. The antibody level dropped to the original prior vaccination. Revaccination at week 14 significantly increased the antibody levels which lasted until the end of the study period at week 18. Stress due to haemonchosis at week 4 however drastically reduced the antibody levels in all groups causing 13 of the animals all of which were from the control group except one from the alum vaccinated group to succumb to pneumonic pasteurellosis. The result indicated that the herd must be re-vaccinated either with spray or alum vacine at 12 week intervals to maintain a protective herd immunity.

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Item Type:	Article
AGROVOC Term:	Sheep
AGROVOC Term:	Pasteurella
AGROVOC Term:	Bacterial diseases
AGROVOC Term:	Pasteurellosis
AGROVOC Term:	Disease control
AGROVOC Term:	Disease prevention
AGROVOC Term:	Vaccination
AGROVOC Term:	Sprays
AGROVOC Term:	Vaccines
AGROVOC Term:	Antibodies
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8829

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