Detection of maternal antibody against Newcastle disease virus in chicks using an indirect immunoperoxidase test


Citation

Awang I. P. R., . and Wan Ahmad Kusairy W. S., . and Abdul Razak J., . Detection of maternal antibody against Newcastle disease virus in chicks using an indirect immunoperoxidase test. pp. 19-23. ISSN 9128-2506

Abstract

An indirect immunoperoxidase test (IIPT) was used to detect maternal antibody against Newcastle disease virus in one to 21 day old Indian River Chicks. The results of the IIPT were simultaneously compared with those of the microtitre version of the haemagglutination inhibition test (HIT). The IIPT could uniformly detect maternal antibody in 100 of the test sera collected at two day intervals between day 1 to day 17; the HIT could only detect varying percentage (5 to 90) of the maternal antibody-positive sera. The IIPT could even detect the antibody until the later age (day 19) of the chicks whereas the HIT could only detect the antibody until day 17. The findings of this study supports the current practice of not vaccinating one-day-old chicks with Newcastle disease virus vaccine as the maternal antibody at high levels may neutralize the introduced vaccine antigens to render the vaccine ineffective.


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Abstract

An indirect immunoperoxidase test (IIPT) was used to detect maternal antibody against Newcastle disease virus in one to 21 day old Indian River Chicks. The results of the IIPT were simultaneously compared with those of the microtitre version of the haemagglutination inhibition test (HIT). The IIPT could uniformly detect maternal antibody in 100 of the test sera collected at two day intervals between day 1 to day 17; the HIT could only detect varying percentage (5 to 90) of the maternal antibody-positive sera. The IIPT could even detect the antibody until the later age (day 19) of the chicks whereas the HIT could only detect the antibody until day 17. The findings of this study supports the current practice of not vaccinating one-day-old chicks with Newcastle disease virus vaccine as the maternal antibody at high levels may neutralize the introduced vaccine antigens to render the vaccine ineffective.

Additional Metadata

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Item Type: Article
AGROVOC Term: Newcastle disease virus
AGROVOC Term: Chicks
AGROVOC Term: Poultry
AGROVOC Term: Maternal immunity
AGROVOC Term: Immunoperoxidase techniques
AGROVOC Term: Haemagglutination inhibition tests
AGROVOC Term: Antibody formation
AGROVOC Term: Antigens
AGROVOC Term: Vaccination
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8886

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