Detection of field Newcastle disease virus by an indirect immunoperoxidase assay


Citation

Rodiah Husin, . and Aini I., . and Abdul Rahim O., . and Awang I. P. R, . and Kamaruddin A. I., . and Ahmad Shafri H., . Detection of field Newcastle disease virus by an indirect immunoperoxidase assay. pp. 129-132. ISSN 9128-2506

Abstract

In adopting indirect immunoperoxidase (IIP) assay for diagnosing animal diseases we compared the performance of an (IIP) assay for diagnosing animal diseases we compared the performance of an IIP assay with a modified virus isolation-haemagglutination and haemagglutination-inhibition (HI) assay using Newcastle disease (ND) as a model. Both assays detected Newcastle disease virus (NDV) in 25 of the 38 tissue specimens from chickens showing signs and lesions of ND. However the IIP assay could detect NDV in the filtrates of the specimens within 40 hours at a much faster speed than by the virus-isolation-haemagglutination and HI assay which took 2 to 28 days.


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Abstract

In adopting indirect immunoperoxidase (IIP) assay for diagnosing animal diseases we compared the performance of an (IIP) assay for diagnosing animal diseases we compared the performance of an IIP assay with a modified virus isolation-haemagglutination and haemagglutination-inhibition (HI) assay using Newcastle disease (ND) as a model. Both assays detected Newcastle disease virus (NDV) in 25 of the 38 tissue specimens from chickens showing signs and lesions of ND. However the IIP assay could detect NDV in the filtrates of the specimens within 40 hours at a much faster speed than by the virus-isolation-haemagglutination and HI assay which took 2 to 28 days.

Additional Metadata

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Item Type: Article
AGROVOC Term: Newcastle disease virus
AGROVOC Term: Animal diseases
AGROVOC Term: Diagnosis
AGROVOC Term: Performance testing
AGROVOC Term: Immunoperoxidase techniques
AGROVOC Term: Haemagglutination inhibition tests
AGROVOC Term: Haemagglutination tests
AGROVOC Term: Chickens
AGROVOC Term: Lesions
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8903

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