Isolation of hyaluronic acid from yellowfin tuna Thunnus albacares (Bonnaterre 1788) eyeball

Sumogod Angeleca, . and Nacional Loda, . and Nillos Mae Grace, . and Yap Encarnacion Emilia, . Isolation of hyaluronic acid from yellowfin tuna Thunnus albacares (Bonnaterre 1788) eyeball. pp. 1-9. ISSN 0116-6514

Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However due to the risk associated with animal and bacterial derived contaminants and the laborious production that tend to compromise the quality of HA research studies have recently shifted to the exploration of HA in marine resources and the enhancement of the protocols for its production. In this study isolation of HA from yellowfin tuna Thunnus albacares (Bonnaterre 1788) eyeball was carried out through standardisation of the different steps in HA isolation including tissue extraction enzymatic hydrolysis precipitation with cetylpiridinium chloride (CPC) - sodium chloride (NaCl) solution filtration and diafiltration precipitation in alkaline hydroalcoholic solution and lyophilisation. Results revealed that the highest HA yield and purity were observed in the indirect treatment wherein the raw material was pre-treated with acetone formaldehyde and sodium acetate solution prior to water extraction and incubation of the mixture for 24 h. Enzymatic hydrolysis revealed that 6 h was the maximum tissue hydrolysis period. Results further showed that the optimum conditions for HA isolation were through the use of 3 CPC:3M NaCl concentration for recovery and fractionation and a 1:3 mL.mL-1 supernatant:ethanol ratio for alcoholic precipitation. The data gathered from this study could help maximise the benefits of the tuna processing waste in the country and may open a new opportunity for more cost-effective production of a valuable bioactive compound from a natural source.

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Abstract

Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However due to the risk associated with animal and bacterial derived contaminants and the laborious production that tend to compromise the quality of HA research studies have recently shifted to the exploration of HA in marine resources and the enhancement of the protocols for its production. In this study isolation of HA from yellowfin tuna Thunnus albacares (Bonnaterre 1788) eyeball was carried out through standardisation of the different steps in HA isolation including tissue extraction enzymatic hydrolysis precipitation with cetylpiridinium chloride (CPC) - sodium chloride (NaCl) solution filtration and diafiltration precipitation in alkaline hydroalcoholic solution and lyophilisation. Results revealed that the highest HA yield and purity were observed in the indirect treatment wherein the raw material was pre-treated with acetone formaldehyde and sodium acetate solution prior to water extraction and incubation of the mixture for 24 h. Enzymatic hydrolysis revealed that 6 h was the maximum tissue hydrolysis period. Results further showed that the optimum conditions for HA isolation were through the use of 3 CPC:3M NaCl concentration for recovery and fractionation and a 1:3 mL.mL-1 supernatant:ethanol ratio for alcoholic precipitation. The data gathered from this study could help maximise the benefits of the tuna processing waste in the country and may open a new opportunity for more cost-effective production of a valuable bioactive compound from a natural source.

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Item Type:	Article
AGROVOC Term:	Hyaluronic acid
AGROVOC Term:	Isolation
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Precipitation
AGROVOC Term:	Filtration
AGROVOC Term:	Tuna
AGROVOC Term:	Thunnus albacares
AGROVOC Term:	Eyes
AGROVOC Term:	Yields
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8942

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