Citation
Siti Zaharah R., . and Noranizan M., . and Son R., . and Roselina K., . and Yusof N. L., . and Koh P. C., . and Nor Hasni H., . Microbiological and physical properties of pennywort (Centella asiatica) leaves using pulsed light technology. pp. 16-27. ISSN 2231-7546
Abstract
Pennywort (Centella asiatica) is a herbaceous vegetable commonly consumed raw as ulam or salad. Consumption of raw leafy green vegetables is one of the pathogenic mechanisms that could cause foodborne outbreaks. The aim of the present work was therefore to investigate the effect of pulsed light (PL) treatment at fluences of 1.5 4.2 6.9 9.6 and 12.3 J/cm on the microbiological and physical quality of pennywort stored at 4 1C. Escherichia coli (E. coli) were inoculated onto the pennywort leaves before being exposed to PL and viewed using scanning electron microscopy (SEM). PL fluences of 6.9 9.6 and 12.3 J/cm significantly reduced the microbial count; however the highest inactivation was obtained by using fluences of 9.6 and 12.3 J/cm. The color of pennywort was not significantly affected by PL treatment applied at lower fluences of 1.5 4.2 and 6.9 J/cm; however at higher fluence 9.6 and 12.3 J/cm the color was affected. PL at 1.5 4.2 6.9 and 9.6 J/cm was able to retain the texture appearance of the leaves. To conclude PL at 6.9 J/cm showed the best fluence to reduce total aerobic mesophilic count while retaining the physical properties of pennywort leaves and extend the shelf life to about four days. The inactivation of E. coli population was significantly higher at PL fluence of 6.9 J/cm. It was observed that PL caused the destruction to the surface of E. colis cell membrane. The reductions of samples inoculated with E. coli were better than those achieved in native microbiota. Furthermore the present work also demonstrated that PL treatment was able to reduce the microbial count on pennywort leaves.
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Abstract
Pennywort (Centella asiatica) is a herbaceous vegetable commonly consumed raw as ulam or salad. Consumption of raw leafy green vegetables is one of the pathogenic mechanisms that could cause foodborne outbreaks. The aim of the present work was therefore to investigate the effect of pulsed light (PL) treatment at fluences of 1.5 4.2 6.9 9.6 and 12.3 J/cm on the microbiological and physical quality of pennywort stored at 4 1C. Escherichia coli (E. coli) were inoculated onto the pennywort leaves before being exposed to PL and viewed using scanning electron microscopy (SEM). PL fluences of 6.9 9.6 and 12.3 J/cm significantly reduced the microbial count; however the highest inactivation was obtained by using fluences of 9.6 and 12.3 J/cm. The color of pennywort was not significantly affected by PL treatment applied at lower fluences of 1.5 4.2 and 6.9 J/cm; however at higher fluence 9.6 and 12.3 J/cm the color was affected. PL at 1.5 4.2 6.9 and 9.6 J/cm was able to retain the texture appearance of the leaves. To conclude PL at 6.9 J/cm showed the best fluence to reduce total aerobic mesophilic count while retaining the physical properties of pennywort leaves and extend the shelf life to about four days. The inactivation of E. coli population was significantly higher at PL fluence of 6.9 J/cm. It was observed that PL caused the destruction to the surface of E. colis cell membrane. The reductions of samples inoculated with E. coli were better than those achieved in native microbiota. Furthermore the present work also demonstrated that PL treatment was able to reduce the microbial count on pennywort leaves.
Additional Metadata
Item Type: | Article |
---|---|
AGROVOC Term: | Leafy vegetables |
AGROVOC Term: | Herbaceous plants |
AGROVOC Term: | Pulsed lighting |
AGROVOC Term: | Microbiological analysis |
AGROVOC Term: | Physical properties |
AGROVOC Term: | Escherichia coli |
AGROVOC Term: | Electron microscopy |
AGROVOC Term: | Sampling |
AGROVOC Term: | Experiments |
AGROVOC Term: | Water analysis |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:54 |
URI: | http://webagris.upm.edu.my/id/eprint/8968 |
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