Preparation antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum arabic-stabilised magnesium orotate nanoparticles


Citation

Kamarudin S., . and Hussain S. A., . and Rozita R., . and Hassani A., . and Abdullah N., . Preparation antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum arabic-stabilised magnesium orotate nanoparticles. pp. 150-159. ISSN 2231-7546

Abstract

The present work investigated the antioxidant properties and antihypertensive activity of magnesium orotate (MgOr) using various established in vitro assays such as -carotene bleaching activity 1 1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide scavenging activity as well as angiotensin converting enzyme (ACE) inhibitory activity. Magnesium orotate nanoparticles (MgOrGANPs) were prepared using the gum arabic (GA) as stabiliser coatings for nanoparticles through freeze-drying method. The in vitro cytoxicity of MgOrGANPs against human breast cancer MCF7 liver cancer HepG2 and colon cancer HT29 was investigated. The nitric oxide (NO) and DPPH scavenging assays of MgOrGANPs showed a dose-dependent trend while 500 and 200 L/mL were significantly more effective than the other concentrations with an IC‚‚ of 89.56 g/mL and 63.22 DPPH scavenging capacity respectively. The exposure of human cancer cells to MgOrGANPs at 1.56 “ 1 000 g/mL using 3-)4 5-dimethylthiazol-2-yl(2 5-diphenyl tetrazolium bromide (MTT) inhibited the growth of cell lines examined in a dose-dependent manner. Hence MgOrGANPs may have great potential to be applied for cancer treatments.


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Abstract

The present work investigated the antioxidant properties and antihypertensive activity of magnesium orotate (MgOr) using various established in vitro assays such as -carotene bleaching activity 1 1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide scavenging activity as well as angiotensin converting enzyme (ACE) inhibitory activity. Magnesium orotate nanoparticles (MgOrGANPs) were prepared using the gum arabic (GA) as stabiliser coatings for nanoparticles through freeze-drying method. The in vitro cytoxicity of MgOrGANPs against human breast cancer MCF7 liver cancer HepG2 and colon cancer HT29 was investigated. The nitric oxide (NO) and DPPH scavenging assays of MgOrGANPs showed a dose-dependent trend while 500 and 200 L/mL were significantly more effective than the other concentrations with an IC‚‚ of 89.56 g/mL and 63.22 DPPH scavenging capacity respectively. The exposure of human cancer cells to MgOrGANPs at 1.56 “ 1 000 g/mL using 3-)4 5-dimethylthiazol-2-yl(2 5-diphenyl tetrazolium bromide (MTT) inhibited the growth of cell lines examined in a dose-dependent manner. Hence MgOrGANPs may have great potential to be applied for cancer treatments.

Additional Metadata

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Item Type: Article
AGROVOC Term: Gum arabic
AGROVOC Term: Polysaccharides
AGROVOC Term: Antioxidants
AGROVOC Term: Antihypertensive agents
AGROVOC Term: Magnesium
AGROVOC Term: Polymers
AGROVOC Term: Cytotoxicity
AGROVOC Term: Angiotensin
AGROVOC Term: Nitric oxide
AGROVOC Term: Enzymes
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8982

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