Citation
Zia M. A., . and Pasha I., . and Zhu M. J., . and Manzoor M. S., . and Shehzad A., . Antioxidant profiling of indigenous oat cultivars with special reference to avenanthramides. pp. 261-269. ISSN 2231-7546
Abstract
Oat (Avena sativa) of the South Asian region particularly Pakistan remains neglected for decades. Oat is unique in terms of the presence of exceptional polyphenolic compounds known as avenanthramides (AVAs). AVAs have therapeutic effects and behave as antioxidative anti-proliferative and vasodilatory agents. The present work was designed to explore the nutritional and antioxidant aspects of oat especially of their phenolic content including AVAs. Three cultivars of oat (S-2000 S-2011 and L-632) were examined for their proximate composition and mineral profile. Conventional solvents (acetone ethanol and methanol) and supercritical carbon dioxide (SC-CO‚‚) were used for polyphenol extraction. Extracts were spectrophotometrically analysed for their phytochemical profile and antioxidant activity. Vital avenanthramides (AVA-A AVA-B and AVA-C) were quantified through HPLC/UV-Vis detector. The data reported highest total phenolic content (222.72 mg GAE/100 g) and total flavonoid content (137.13 mg QE/100 g) in S-2011 with maximum antioxidant activity (1 1-diphenyl-2-picrylhydrazyl IC‚‚ 12.38 mg/ml and ferric reducing antioxidant power 39.98 mol/g). This highest antioxidant potential was examined in supercritical extract while methanol evaluated the best among conventional solvents. Best extractions were further analysed with HPLC for the quantification of AVAs. SC-CO‚‚ extraction recorded maximum concentrations of AVA-A (137.84 g/g) AVA-B (105.10 g/g) and AVA-C (119.86 g/g) in S-2011 cultivar. In conclusion oat is the rich source of antioxidants with the presence of exceptional AVAs.
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Abstract
Oat (Avena sativa) of the South Asian region particularly Pakistan remains neglected for decades. Oat is unique in terms of the presence of exceptional polyphenolic compounds known as avenanthramides (AVAs). AVAs have therapeutic effects and behave as antioxidative anti-proliferative and vasodilatory agents. The present work was designed to explore the nutritional and antioxidant aspects of oat especially of their phenolic content including AVAs. Three cultivars of oat (S-2000 S-2011 and L-632) were examined for their proximate composition and mineral profile. Conventional solvents (acetone ethanol and methanol) and supercritical carbon dioxide (SC-CO‚‚) were used for polyphenol extraction. Extracts were spectrophotometrically analysed for their phytochemical profile and antioxidant activity. Vital avenanthramides (AVA-A AVA-B and AVA-C) were quantified through HPLC/UV-Vis detector. The data reported highest total phenolic content (222.72 mg GAE/100 g) and total flavonoid content (137.13 mg QE/100 g) in S-2011 with maximum antioxidant activity (1 1-diphenyl-2-picrylhydrazyl IC‚‚ 12.38 mg/ml and ferric reducing antioxidant power 39.98 mol/g). This highest antioxidant potential was examined in supercritical extract while methanol evaluated the best among conventional solvents. Best extractions were further analysed with HPLC for the quantification of AVAs. SC-CO‚‚ extraction recorded maximum concentrations of AVA-A (137.84 g/g) AVA-B (105.10 g/g) and AVA-C (119.86 g/g) in S-2011 cultivar. In conclusion oat is the rich source of antioxidants with the presence of exceptional AVAs.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Oats |
AGROVOC Term: | Avena sativa |
AGROVOC Term: | Antioxidants |
AGROVOC Term: | Ethanol |
AGROVOC Term: | Methanol |
AGROVOC Term: | Acetone |
AGROVOC Term: | Phenolic content |
AGROVOC Term: | Moisture content |
AGROVOC Term: | Polyphenols |
AGROVOC Term: | Statistical analysis |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:54 |
URI: | http://webagris.upm.edu.my/id/eprint/8993 |
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