Screening identification and application of deoxynivalenol-detoxifying bacteria from mildewed alfalfa silage

Yu S. Y., . and Li W., . and Cheng P., . and Zhang J. B., . Screening identification and application of deoxynivalenol-detoxifying bacteria from mildewed alfalfa silage. pp. 350-356. ISSN 2231-7546

Deoxynivalenol (DON) is very harmful to food and feed. Biological detoxification is an effective method to reduce the harmful effect and ensure safety of food and feed. In order to screen the strains that can detoxify DON 10 samples were selected from the mouldy alfalfa silage; and the LB NA and MRS media with DON were used for primary screening and re-screening. The detoxification rate of DON was measured and calculated by ELISA method. The strains with high detoxification rate of DON were identified by morphology and 16S rDNA sequence analysis. The selected strains were combined and used to ferment the bran and the detoxification efficiency of DON in the bran was then determined. Sixteen strains with the ability of detoxification were screened and the results showed that the highest detoxification rate was 66.75. Three strains with high detoxification rate were isolated and identified as Bacillus subtilis Pediococcus acidilactici and Lactobacillus plantarum. The combination of B. subtilis and L. plantarum yielded the highest efficiency of DON detoxification in bran which could reach to 75.98 in 96 h.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(02)%202020/16%20-...

Abstract

Deoxynivalenol (DON) is very harmful to food and feed. Biological detoxification is an effective method to reduce the harmful effect and ensure safety of food and feed. In order to screen the strains that can detoxify DON 10 samples were selected from the mouldy alfalfa silage; and the LB NA and MRS media with DON were used for primary screening and re-screening. The detoxification rate of DON was measured and calculated by ELISA method. The strains with high detoxification rate of DON were identified by morphology and 16S rDNA sequence analysis. The selected strains were combined and used to ferment the bran and the detoxification efficiency of DON in the bran was then determined. Sixteen strains with the ability of detoxification were screened and the results showed that the highest detoxification rate was 66.75. Three strains with high detoxification rate were isolated and identified as Bacillus subtilis Pediococcus acidilactici and Lactobacillus plantarum. The combination of B. subtilis and L. plantarum yielded the highest efficiency of DON detoxification in bran which could reach to 75.98 in 96 h.

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Item Type:	Article
AGROVOC Term:	Deoxynivalenol
AGROVOC Term:	Alfalfa
AGROVOC Term:	Silage
AGROVOC Term:	Fermentation
AGROVOC Term:	Detoxification
AGROVOC Term:	Mycotoxins
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Screening tests
AGROVOC Term:	Identification
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9018

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