Characterisation of antioxidant anti-inflammatory and immunomodulatory activities of polysaccharides derived from Duchesnea indica (Andrews) Focke

Li B., . and Yu X., . and Xiong Y., . and Xiang B., . and Long M., . and He Q., . Characterisation of antioxidant anti-inflammatory and immunomodulatory activities of polysaccharides derived from Duchesnea indica (Andrews) Focke. pp. 376-384. ISSN 2231-7546

Duchesnea indica (Andrews) Focke is a traditional Chinese medicine used as antiviral astringent anti-inflammatory and antitumor agent in various clinical settings. In the present work D. indica polysaccharides (DIPs) were assessed following hot water extraction ethanol precipitation and deproteinisation. The molecular weight of DIPs was estimated by GPC as 3.018 10� Da. HPLC results indicated that DIPs were mainly composed of D-mannose D-ribose L-rhamnose D-glucosamine D-glucuronic acid D-galacturonic acid D-glucose D-galactose and D-arabinose in a molar ratio of 1.54:0.50:3.83:1.56:1.33:3.39:23.69:15.26:8.86 respectively. With regard to antioxidant activities DIPs had a strong radical scavenging capacity and protective effect against DNA damage. Furthermore DIPs presented a significant anti-inflammatory ability in vivo using both xylene-induced ear oedema and -carrageenan-induced paw oedema models. Lastly DIPs significantly inhibited generation of NO and cytokines (TNF-a and IL-6) in LPS-induced RAW264.7 macrophages. These data strongly indicate that DIPs could be explored as potentially natural antioxidant anti-inflammatory and immunomodulatory agents.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(02)%202020/19%20-...

Abstract

Duchesnea indica (Andrews) Focke is a traditional Chinese medicine used as antiviral astringent anti-inflammatory and antitumor agent in various clinical settings. In the present work D. indica polysaccharides (DIPs) were assessed following hot water extraction ethanol precipitation and deproteinisation. The molecular weight of DIPs was estimated by GPC as 3.018 10� Da. HPLC results indicated that DIPs were mainly composed of D-mannose D-ribose L-rhamnose D-glucosamine D-glucuronic acid D-galacturonic acid D-glucose D-galactose and D-arabinose in a molar ratio of 1.54:0.50:3.83:1.56:1.33:3.39:23.69:15.26:8.86 respectively. With regard to antioxidant activities DIPs had a strong radical scavenging capacity and protective effect against DNA damage. Furthermore DIPs presented a significant anti-inflammatory ability in vivo using both xylene-induced ear oedema and -carrageenan-induced paw oedema models. Lastly DIPs significantly inhibited generation of NO and cytokines (TNF-a and IL-6) in LPS-induced RAW264.7 macrophages. These data strongly indicate that DIPs could be explored as potentially natural antioxidant anti-inflammatory and immunomodulatory agents.

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Item Type:	Article
AGROVOC Term:	Traditional medicines
AGROVOC Term:	Antioxidants
AGROVOC Term:	Antiinflammatory agents
AGROVOC Term:	Polysaccharides
AGROVOC Term:	Extraction
AGROVOC Term:	Monosaccharides
AGROVOC Term:	Spectroscopy
AGROVOC Term:	Experimental design
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Analysis of variance
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9020

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