Establishment of Pasteurella haemolytica A2 in the lungs of goats following intratracheal exposure

Salim N. B., . and Maswati M. A., . and Sheikh Omar A. R., . and M. Zamri Saad, . and Effendy A. W. M., . Establishment of Pasteurella haemolytica A2 in the lungs of goats following intratracheal exposure. pp. 15-19. ISSN 9128-2506

Twenty healthy goats were divided into three groups of eight goats in each of the first two groups and four goats in group 3. All goats were transport stressed by road before goats in group 1 were challenged intratracheally with Pasteurella haemolytica A2 isolated earlier from the nasal mucosa of a healthy goat and group 2 with P. haemolytica A2 isolated from peumonic lungs of a goat. Group 3 was the uninfected control. The goats were serially slaughtered at 1 4 7 and 11 days post challenge to study the baterial establishment and to compare the extent of lung lesions produced by the two isolates. The gross lung lesions showed significant (P0.05) and progressive development from an average of 6.3 2.1 per cent at 24h post challenge to 26.8 13.1 per cent after 11 days of challenge. P. haemolytica A2 isolated from lungs consistently produced insignificantly (P0.05) more extensive lesions compared to P. haemolytica A2 isolated from the nasal mucosa. There were small numbers of neutrophils present in the lung washing fluid at 24h post challenge before a mixture of neutrophils and macrophages with phagocytic activity was observed at day 4 post challenge. At day 7 post challenge goats that showed more severe lung lesions had numerous bacteria cells in the lung either floating freely in the alveolar spaces or attached to the pneumocytes. At day 11 post challenge the bacteria had penetrated the cell wall and were found in tha vacuoles of pneumocytes.

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Abstract

Twenty healthy goats were divided into three groups of eight goats in each of the first two groups and four goats in group 3. All goats were transport stressed by road before goats in group 1 were challenged intratracheally with Pasteurella haemolytica A2 isolated earlier from the nasal mucosa of a healthy goat and group 2 with P. haemolytica A2 isolated from peumonic lungs of a goat. Group 3 was the uninfected control. The goats were serially slaughtered at 1 4 7 and 11 days post challenge to study the baterial establishment and to compare the extent of lung lesions produced by the two isolates. The gross lung lesions showed significant (P0.05) and progressive development from an average of 6.3 2.1 per cent at 24h post challenge to 26.8 13.1 per cent after 11 days of challenge. P. haemolytica A2 isolated from lungs consistently produced insignificantly (P0.05) more extensive lesions compared to P. haemolytica A2 isolated from the nasal mucosa. There were small numbers of neutrophils present in the lung washing fluid at 24h post challenge before a mixture of neutrophils and macrophages with phagocytic activity was observed at day 4 post challenge. At day 7 post challenge goats that showed more severe lung lesions had numerous bacteria cells in the lung either floating freely in the alveolar spaces or attached to the pneumocytes. At day 11 post challenge the bacteria had penetrated the cell wall and were found in tha vacuoles of pneumocytes.

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Item Type:	Article
AGROVOC Term:	Pasteurella haemolytica
AGROVOC Term:	Lungs
AGROVOC Term:	Goats
AGROVOC Term:	Feed supplements
AGROVOC Term:	Experiments
AGROVOC Term:	Clinical examination
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Electron microscopy
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9039

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