The role of Nannochloropsis sp. Methanolic extract in reducing hydrogen peroxide-induced DNA damage in L929 cell line

Haziq Ahmad Hazwan Zainoddin, . and Eshaifol Azam Omar, . and Nik Nur Syazni Nik Mohamed Kamal, . and Wan Adnan Wan Omar, . The role of Nannochloropsis sp. Methanolic extract in reducing hydrogen peroxide-induced DNA damage in L929 cell line. pp. 81-90. ISSN 1511-3701

Nannochloropsis sp. is one of the microalgae that produces important antioxidants. We postulate that the presence of phenolic compounds in the methanolic extract of Nannochloropsis sp. exhibits preventive effect against DNA damage induced by 100 M concentration of hydrogen peroxide (H‚‚O‚‚) in L929 cells. High performance liquid chromatography (HPLC) analysis revealed that Nannochloropsis sp. methanolic extract contained antioxidant compounds such as p-coumaric acid caffeic acid naringenin and hesperitin. In this study we utilised comet assay to measure the activity of DNA damage induced by H‚‚O‚‚ in L929 cells and the preventive effect of Nannochloropsis sp. Methanolic extract and caffeic acid. After 24 hours of treatment 100 M of H‚‚O‚‚ induced 64.4 4.6 of DNA damage in L929 cells compared with the untreated cells (p 0.05). Interestingly the scale of DNA damage induced by the H‚‚O‚‚ was reduced to 46.3 12.7 when treated with 0.4 mg/mL of Nannochloropsis sp. methanolic extract (p 0.01). The DNA damage was further reduced to 7.3 2.9 when treated with 20 M of caffeic acid (p 0.01) a compound found in the microalgae extract. In conclusion Nannochloropsis sp. methanolic extract which contained phenolic compounds was able to protect L929 normal cells from oxidative DNA damage and therefore may confer protection from genetic mutation and cancer formation.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/pjtas/browse/regul...

Abstract

Nannochloropsis sp. is one of the microalgae that produces important antioxidants. We postulate that the presence of phenolic compounds in the methanolic extract of Nannochloropsis sp. exhibits preventive effect against DNA damage induced by 100 M concentration of hydrogen peroxide (H‚‚O‚‚) in L929 cells. High performance liquid chromatography (HPLC) analysis revealed that Nannochloropsis sp. methanolic extract contained antioxidant compounds such as p-coumaric acid caffeic acid naringenin and hesperitin. In this study we utilised comet assay to measure the activity of DNA damage induced by H‚‚O‚‚ in L929 cells and the preventive effect of Nannochloropsis sp. Methanolic extract and caffeic acid. After 24 hours of treatment 100 M of H‚‚O‚‚ induced 64.4 4.6 of DNA damage in L929 cells compared with the untreated cells (p 0.05). Interestingly the scale of DNA damage induced by the H‚‚O‚‚ was reduced to 46.3 12.7 when treated with 0.4 mg/mL of Nannochloropsis sp. methanolic extract (p 0.01). The DNA damage was further reduced to 7.3 2.9 when treated with 20 M of caffeic acid (p 0.01) a compound found in the microalgae extract. In conclusion Nannochloropsis sp. methanolic extract which contained phenolic compounds was able to protect L929 normal cells from oxidative DNA damage and therefore may confer protection from genetic mutation and cancer formation.

[error in script]

Item Type:	Article
AGROVOC Term:	Algae
AGROVOC Term:	Microbiological analysis
AGROVOC Term:	High performance liquid chromatography
AGROVOC Term:	Extracts
AGROVOC Term:	Methanol
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	Caffeic acid
AGROVOC Term:	Coumaric acids
AGROVOC Term:	Antioxidants
AGROVOC Term:	DNA
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9078

Actions (login required)

View Item