Suitability of PSA (Primary Secondary Amines) and Z-Sep as clean-up sorbents in AOAC official method 2007.01 for analysis of trifloxystrobin and CGA 321113 residue in dragon fruit star fruit and watermelon

N. Chai Keong, . and Siti Hajar H., . and Khairatul A. M., . Suitability of PSA (Primary Secondary Amines) and Z-Sep as clean-up sorbents in AOAC official method 2007.01 for analysis of trifloxystrobin and CGA 321113 residue in dragon fruit star fruit and watermelon. pp. 1-10. ISSN 1394-9829

Two types of clean-up sorbents namely PSA (Primary Secondary Amines) and Z-Sep were tested for reliability in AOAC official method 2007.01 for generating accurate results for analysis of trifloxystrobin and CGA 321113 (trifloxystrobinmetabolite) in dragon fruit star fruit and watermelon. A known amount of both analytes was fortified onto separate analytical portions of dragon fruit star fruit and watermelon respectively followed by an extraction clean-up procedure using either PSA or Z-Sep. The extracts were analysed by Liquid Chromatography Tandem Mass Spectrometry (LCMS-MS) analysis. Measured concentrations were compared with a theoretical fortified value in order to determine its recovery. Results indicated that both PSA and Z-Sep generated acceptable recovery of trifloxystrobin ranging from 76.8 “ 107.7. However when PSA alone was used as the clean-up material significant loss of CGA 321113 was deduced from low average recovery values in dragon fruit (47.1) star fruit (57.4) and watermelon (31.7) for fortification level of 0.01 mg/kg. For higher fortification level of 0.1 mg/kg lower average recovery values of CGA 321113 were reported in dragon fruit (23.9) star fruit (15.4) and watermelon (14.6). In all the studied fruits Z-Sep showed recoveries of CGA 321113 within the range of 80.4“ 124.2 at 0.01 and 0.1 mg/kg fortification levels indicating no significant loss during the clean-up procedure. Based on two types of residue definitions (MRL compliance and dietary risk assessment) it can be concluded that for analysis of CGA 321113 Z-Sep should be used as the clean-up sorbent in the clean-up procedure.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/48-1/PSA.pdf

Abstract

Two types of clean-up sorbents namely PSA (Primary Secondary Amines) and Z-Sep were tested for reliability in AOAC official method 2007.01 for generating accurate results for analysis of trifloxystrobin and CGA 321113 (trifloxystrobinmetabolite) in dragon fruit star fruit and watermelon. A known amount of both analytes was fortified onto separate analytical portions of dragon fruit star fruit and watermelon respectively followed by an extraction clean-up procedure using either PSA or Z-Sep. The extracts were analysed by Liquid Chromatography Tandem Mass Spectrometry (LCMS-MS) analysis. Measured concentrations were compared with a theoretical fortified value in order to determine its recovery. Results indicated that both PSA and Z-Sep generated acceptable recovery of trifloxystrobin ranging from 76.8 “ 107.7. However when PSA alone was used as the clean-up material significant loss of CGA 321113 was deduced from low average recovery values in dragon fruit (47.1) star fruit (57.4) and watermelon (31.7) for fortification level of 0.01 mg/kg. For higher fortification level of 0.1 mg/kg lower average recovery values of CGA 321113 were reported in dragon fruit (23.9) star fruit (15.4) and watermelon (14.6). In all the studied fruits Z-Sep showed recoveries of CGA 321113 within the range of 80.4“ 124.2 at 0.01 and 0.1 mg/kg fortification levels indicating no significant loss during the clean-up procedure. Based on two types of residue definitions (MRL compliance and dietary risk assessment) it can be concluded that for analysis of CGA 321113 Z-Sep should be used as the clean-up sorbent in the clean-up procedure.

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Item Type:	Article
AGROVOC Term:	Amines
AGROVOC Term:	analysis
AGROVOC Term:	Star fruit
AGROVOC Term:	Watermelons
AGROVOC Term:	Analytical methods
AGROVOC Term:	Extraction
AGROVOC Term:	Sampling
AGROVOC Term:	Experiments
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Residues
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9090

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