Protein extracted from Clitoria ternatea modulates genes related to diabetes in vivo

Suwanto A., . and Gunawan A. G., . and Yanti, . and Minelko M., . and Ali S., . Protein extracted from Clitoria ternatea modulates genes related to diabetes in vivo. pp. 610-617. ISSN 2231-7546

Butterfly pea (Clitoria ternatea) is an indigenous plant from Moluccas in Indonesia. Due to its pharmacological properties the flower of this plant is also used as a medicine in Ayurvedic practices in India. The present work was aimed to determine the antidiabetic activity of C. ternatea protein extract (PCT) via the inhibition of -amylase activity in vitro and the modulation of genes related to diabetes in diabetic mice in vivo. C. ternatea flower protein extract was prepared using isoprecipitation and its efficacy as an antidiabetic candidate was tested in vitro through -amylase inhibition assay and in vivo through the measurement of blood glucose level and diabetes-related genes expression including those that encode for peroxisome proliferator-activated receptors gamma (PPAR) glucose transporter 2 (Glut2) transcription factor 7-like 2 (Tcf7l2) calpain-10 (Capn10) and monocyte chemoattractant protein 1 (MCP1) in alloxan-induced diabetic mice. PCT showed -amylase inhibition in a dose-dependent manner. The highest -amylase inhibition (20.63) was observed at 1 mg/mL of PCT. The in vivo study showed that PCT decreased the blood glucose level in alloxan-induced diabetic mice. PCT also altered the expression of diabetes-related genes. In adipose and skeletal muscle tissues PCT upregulated the gene expression of PPAR and Tcf7l2 meanwhile MCP1 expression was downregulated. In the pancreas PCT upregulated the gene expression of Glut2 Capn10 and Tcf7l2. These results indicate that PCT is a potential antidiabetic agent for the treatment of type 2 diabetes.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(04)%202020/DONE%2...

Abstract

Butterfly pea (Clitoria ternatea) is an indigenous plant from Moluccas in Indonesia. Due to its pharmacological properties the flower of this plant is also used as a medicine in Ayurvedic practices in India. The present work was aimed to determine the antidiabetic activity of C. ternatea protein extract (PCT) via the inhibition of -amylase activity in vitro and the modulation of genes related to diabetes in diabetic mice in vivo. C. ternatea flower protein extract was prepared using isoprecipitation and its efficacy as an antidiabetic candidate was tested in vitro through -amylase inhibition assay and in vivo through the measurement of blood glucose level and diabetes-related genes expression including those that encode for peroxisome proliferator-activated receptors gamma (PPAR) glucose transporter 2 (Glut2) transcription factor 7-like 2 (Tcf7l2) calpain-10 (Capn10) and monocyte chemoattractant protein 1 (MCP1) in alloxan-induced diabetic mice. PCT showed -amylase inhibition in a dose-dependent manner. The highest -amylase inhibition (20.63) was observed at 1 mg/mL of PCT. The in vivo study showed that PCT decreased the blood glucose level in alloxan-induced diabetic mice. PCT also altered the expression of diabetes-related genes. In adipose and skeletal muscle tissues PCT upregulated the gene expression of PPAR and Tcf7l2 meanwhile MCP1 expression was downregulated. In the pancreas PCT upregulated the gene expression of Glut2 Capn10 and Tcf7l2. These results indicate that PCT is a potential antidiabetic agent for the treatment of type 2 diabetes.

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Item Type:	Article
AGROVOC Term:	Clitoria ternatea
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Proteins
AGROVOC Term:	Diabetes mellitus
AGROVOC Term:	Human diseases
AGROVOC Term:	Extraction
AGROVOC Term:	Flowers
AGROVOC Term:	Genes
AGROVOC Term:	Gene expression
AGROVOC Term:	Animal models
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9231

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