Antioxidant and antidiabetic properties of Phyllanthus acidus (L.) Skeels ethanolic seed extract

Chigurupati S., . Antioxidant and antidiabetic properties of Phyllanthus acidus (L.) Skeels ethanolic seed extract. pp. 775-782. ISSN 2231-7546

Oxidative stress is an important part of diabetic physiopathology; therefore naturally derived antioxidants as therapeutic agents are needed. The present work examined the antioxidant and antidiabetic potentials of seed extract of Phyllanthus acidus (L.) Skeels. Ethanolic seed extract of P. acidus (PSE) was prepared using Soxhlet extraction. Preliminary phytochemical analysis total phenolic (TPC) and total flavonoid (TFC) contents of PSE were determined. The in vitro inhibitory effects of PSE on 1 1-diphenyl-2 picrylhydrazyl (DPPH) 2 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) free radicals -amylase and -glucosidase enzyme inhibitory activities were also assessed. Acute oral toxicity of PSE was carried out in female Sprague Dawley (SD) rats. PSEs hypoglycaemic effect was demonstrated in normoglycemic and streptozotocin-induced (STZ 55 mg/kg ip) diabetic rats. The TPC and TFC were found to be 3.19 mg of gallic acid equivalent/g (GAE/g) and 2.58 mg of quercetin equivalent/g of extract (QE/g) respectively. The inhibitory concentration 50 (IC‚‚) values based on the inhibition of DPPH (IC‚‚) 28.26 0.39 g/mL) ABTS (IC‚‚) 23.44 0.48 g/mL) radicals -amylase (IC‚‚) 26.83 0.39 g/mL) and -glucosidase (IC‚‚) 19.12 0.26 g/mL) enzymes were generally lower which demonstrated their potent antioxidant and antidiabetic effects respectively. Based on the acute oral toxicity test two doses (100 and 200 mg/kg) of PSE were tested in normoglycemic rats. A significant (p 0.01) fall in blood glucose was observed at 8 and 12 h after PSE (200 mg/kg) treatment. The same dose was tested in STZ rats and compared with glibenclamide (5 mg/kg). A significant (p 0.01 and p 0.001) decrease in the blood glucose level was observed after 8 and 12 h of PSE treatment respectively. These findings suggest that PSE could be used as an active ingredient in herbal formulations to manage diabetes mellitus.

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Abstract

Oxidative stress is an important part of diabetic physiopathology; therefore naturally derived antioxidants as therapeutic agents are needed. The present work examined the antioxidant and antidiabetic potentials of seed extract of Phyllanthus acidus (L.) Skeels. Ethanolic seed extract of P. acidus (PSE) was prepared using Soxhlet extraction. Preliminary phytochemical analysis total phenolic (TPC) and total flavonoid (TFC) contents of PSE were determined. The in vitro inhibitory effects of PSE on 1 1-diphenyl-2 picrylhydrazyl (DPPH) 2 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) free radicals -amylase and -glucosidase enzyme inhibitory activities were also assessed. Acute oral toxicity of PSE was carried out in female Sprague Dawley (SD) rats. PSEs hypoglycaemic effect was demonstrated in normoglycemic and streptozotocin-induced (STZ 55 mg/kg ip) diabetic rats. The TPC and TFC were found to be 3.19 mg of gallic acid equivalent/g (GAE/g) and 2.58 mg of quercetin equivalent/g of extract (QE/g) respectively. The inhibitory concentration 50 (IC‚‚) values based on the inhibition of DPPH (IC‚‚) 28.26 0.39 g/mL) ABTS (IC‚‚) 23.44 0.48 g/mL) radicals -amylase (IC‚‚) 26.83 0.39 g/mL) and -glucosidase (IC‚‚) 19.12 0.26 g/mL) enzymes were generally lower which demonstrated their potent antioxidant and antidiabetic effects respectively. Based on the acute oral toxicity test two doses (100 and 200 mg/kg) of PSE were tested in normoglycemic rats. A significant (p 0.01) fall in blood glucose was observed at 8 and 12 h after PSE (200 mg/kg) treatment. The same dose was tested in STZ rats and compared with glibenclamide (5 mg/kg). A significant (p 0.01 and p 0.001) decrease in the blood glucose level was observed after 8 and 12 h of PSE treatment respectively. These findings suggest that PSE could be used as an active ingredient in herbal formulations to manage diabetes mellitus.

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Item Type:	Article
AGROVOC Term:	Phyllanthus acidus
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Antioxidants
AGROVOC Term:	Seed extracts
AGROVOC Term:	Plant extracts
AGROVOC Term:	Chemical composition
AGROVOC Term:	Flavonoids
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	Free radicals
AGROVOC Term:	Rats
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9288

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