MRN Complex and ATM Kinase Inhibitors Impacts towards UVC-Treated Zebrafish Embryonic Development

Shazrul Fazry, . and Nur Hidayah Jamar, . and Nor Azian Abdul Murad, . and Nik Marzuki Sidik, . and Herryawan Ryadi Eziwar Dyari, . and Nahid Khalili, . and Malina Kumaran, . and Lim Yi Chieh, . and Law Douglas, . and Ibrahim Mahmood, . MRN Complex and ATM Kinase Inhibitors Impacts towards UVC-Treated Zebrafish Embryonic Development. pp. 1615-1624. ISSN 2231-8526

The zebrafish (Danio rerio) has become a prevailing vertebrate model for developmental biology studies due to its ease of care rapid embryogenesis stages development and translucent embryos. In this studies ATM Kinase and MRN complex role as DNA damage response proteins during embryogenesis was examined by using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). To create DNA lesions in zebrafish embryos at mid-blastula transition (MBT) stage were exposed to inhibitors (Mirin Ku60019 or Ku55933) and later exposed to UVC irradiation wavelength of between 100 to 280 nm. Hatching but with visible physical deformation was observed for embryos treated with Mirin Ku60019 or Ku55933 and UVC exposure at concentration of 3M 1.5 Nm and 3nM or lower respectively up to 72 hours-post fertilisation (hpf). On the other hand no deformities were observed for all control as well as mock treated embryos. This study confirmed that DNA damage response proteins are crucial during embryo development to prevent undesired abnormal biological development. Thus it is proven that protein inhibitors are a cheaper alternative in valuating specific protein roles during embryogenesis compared to both genomic and transcription modification tools.

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Abstract

The zebrafish (Danio rerio) has become a prevailing vertebrate model for developmental biology studies due to its ease of care rapid embryogenesis stages development and translucent embryos. In this studies ATM Kinase and MRN complex role as DNA damage response proteins during embryogenesis was examined by using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). To create DNA lesions in zebrafish embryos at mid-blastula transition (MBT) stage were exposed to inhibitors (Mirin Ku60019 or Ku55933) and later exposed to UVC irradiation wavelength of between 100 to 280 nm. Hatching but with visible physical deformation was observed for embryos treated with Mirin Ku60019 or Ku55933 and UVC exposure at concentration of 3M 1.5 Nm and 3nM or lower respectively up to 72 hours-post fertilisation (hpf). On the other hand no deformities were observed for all control as well as mock treated embryos. This study confirmed that DNA damage response proteins are crucial during embryo development to prevent undesired abnormal biological development. Thus it is proven that protein inhibitors are a cheaper alternative in valuating specific protein roles during embryogenesis compared to both genomic and transcription modification tools.

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Item Type:	Article
AGROVOC Term:	Danio rerio
AGROVOC Term:	Kinases
AGROVOC Term:	Embryogenesis
AGROVOC Term:	Experimentation
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Phenotypes
AGROVOC Term:	Fertilization
AGROVOC Term:	Animal embryos
AGROVOC Term:	Biological development
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9389

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