Cytotoxicity and antimicrobial activity screening of Gunung Jerai plants

Asma Dazni Danial and Tan, A. N. and Ooi, W. L. and Beh, S. Y. and Neoh, B. K. and Muhd. Farish Kamaludin and Mohd. Amiron Ersad and Mohd. Zairey Azwan Mohd. Zain and Saifuddin Samsudin and Tan, P. S. (2006) Cytotoxicity and antimicrobial activity screening of Gunung Jerai plants. In: Hutan simpan Gunung Jerai, Kedah: pengurusan, persekitaran fizikal dan kepelbagaian biologi. Siri kepelbagaian biologi hutan (6). Jabatan Perhutanan Semenanjung Malaysia, Kuala Lumpur, Malaysia, pp. 283-291. ISBN 9839269402

A total of 48 species belonging to 30 families were randomly collected and subjected to cytotoxicity and antimicrobial screening. Several plants were also collected and cultivated at SDTC for further studies. Cytotoxicity screening was performed using the MTT assay method using human breast cancer cell lines, MCF-7 and Tamoxifen, an anticancer drug, as a positive control for comparison. The MTT assay suggests that 15% of plant species tested contains compounds that are cytotoxic to T-47D cells. However, none of the plant extract tested was cytotoxic towards MCF- 7 cells. For the antimicrobial screening, a total of eight panel organisms comprising five non-pathogenic microbes, Escherichia coli ATCC 25922, Baker's yeast, Saccharomyces cereviciae, Aspergillus niger, Bacillus sphaericus, Pseudomonas aeruginosa and three from the pathogenic group, methicillin-resistant Staphylococcus aureus, MRSA S138, Escherichia coli O157:H7 and vancomycin-resistant Enterococcus, VRE were subjected to the ADT assay method whereby the appearance of an inhibition zone would indicate the presence of antimicrobial activity. All plant species showed an inhibition towards some of the panel organisms tested, except Didymocarpus malayana (Gesneriaceae) and Dehaasia sp. (Lauraceae), which did not show any signs of inhibition. Further investigation will be performed on these promising plant extracts.

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Abstract

A total of 48 species belonging to 30 families were randomly collected and subjected to cytotoxicity and antimicrobial screening. Several plants were also collected and cultivated at SDTC for further studies. Cytotoxicity screening was performed using the MTT assay method using human breast cancer cell lines, MCF-7 and Tamoxifen, an anticancer drug, as a positive control for comparison. The MTT assay suggests that 15% of plant species tested contains compounds that are cytotoxic to T-47D cells. However, none of the plant extract tested was cytotoxic towards MCF- 7 cells. For the antimicrobial screening, a total of eight panel organisms comprising five non-pathogenic microbes, Escherichia coli ATCC 25922, Baker's yeast, Saccharomyces cereviciae, Aspergillus niger, Bacillus sphaericus, Pseudomonas aeruginosa and three from the pathogenic group, methicillin-resistant Staphylococcus aureus, MRSA S138, Escherichia coli O157:H7 and vancomycin-resistant Enterococcus, VRE were subjected to the ADT assay method whereby the appearance of an inhibition zone would indicate the presence of antimicrobial activity. All plant species showed an inhibition towards some of the panel organisms tested, except Didymocarpus malayana (Gesneriaceae) and Dehaasia sp. (Lauraceae), which did not show any signs of inhibition. Further investigation will be performed on these promising plant extracts.

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Item Type:	Book Section
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43000 UPM Serdang, Malaysia.Call Number QH77 M3K4973
AGROVOC Term:	cytotoxicity
AGROVOC Term:	antimicrobials
AGROVOC Term:	screening
AGROVOC Term:	data collection
AGROVOC Term:	expedition
AGROVOC Term:	sampling
AGROVOC Term:	mountains
Geographical Term:	Malaysia
Depositing User:	Ms. Azariah Hashim
Date Deposited:	26 Aug 2024 05:54
Last Modified:	26 Aug 2024 05:54
URI:	http://webagris.upm.edu.my/id/eprint/940

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