Development of co-immobilised enzymes amperometric biosensor for the determination of triglycerides in coconut milk

Chittibabu S., . and Auddy I., . and Shanmugasundaram S., . and Manoj D., . and Nimbkar S., . Development of co-immobilised enzymes amperometric biosensor for the determination of triglycerides in coconut milk. pp. 875-882. ISSN 22317546

Enzyme biosensor was developed to determine the triglycerides present in coconut milk. Lipase glycerol-3-phosphate oxidase (GPO) and glycerol kinase (GK) were co-immobilised on the gelatine membrane and coated on the surface of the working electrode. The standardisation of the biosensor was carried out at different standard triolein solutions. The effect of gelatine concentration glutaraldehyde concentration and pH of reaction buffer were optimised. It was found that 45 mg gelatine 2.5 glutaraldehyde concentration and pH 7.0 yielded the best results. The developed biosensor could detect the triglyceride concentration between 0.3 and 1.3 mM. Triglyceride content in coconut milk was determined using the developed amperometric biosensor method and the results obtained were compared with the triglyceride analysis in coconut milk by ultra-high performance liquid chromatography (UHPLC) method. It was found that the correlation between the results of the two methods was 97. Apart from these thermal stability and sensitivity loss on storage of the working electrode was also studied. The working electrode lost 48 of its activity in 4 h when maintained at 40C and lost 57.7 of its activity after 30 d when stored at 4C.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(05)%202020/DONE%2...

Abstract

Enzyme biosensor was developed to determine the triglycerides present in coconut milk. Lipase glycerol-3-phosphate oxidase (GPO) and glycerol kinase (GK) were co-immobilised on the gelatine membrane and coated on the surface of the working electrode. The standardisation of the biosensor was carried out at different standard triolein solutions. The effect of gelatine concentration glutaraldehyde concentration and pH of reaction buffer were optimised. It was found that 45 mg gelatine 2.5 glutaraldehyde concentration and pH 7.0 yielded the best results. The developed biosensor could detect the triglyceride concentration between 0.3 and 1.3 mM. Triglyceride content in coconut milk was determined using the developed amperometric biosensor method and the results obtained were compared with the triglyceride analysis in coconut milk by ultra-high performance liquid chromatography (UHPLC) method. It was found that the correlation between the results of the two methods was 97. Apart from these thermal stability and sensitivity loss on storage of the working electrode was also studied. The working electrode lost 48 of its activity in 4 h when maintained at 40C and lost 57.7 of its activity after 30 d when stored at 4C.

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Item Type:	Article
AGROVOC Term:	Cocos nucifera
AGROVOC Term:	Coconuts
AGROVOC Term:	Coconut oil
AGROVOC Term:	Biosensors
AGROVOC Term:	Enzymes
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Glycerol
AGROVOC Term:	Gelatin
AGROVOC Term:	Analytical methods
AGROVOC Term:	Heat stability
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9403

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