Resveratrol-enriched transgenic rice callus extract (IS526) causes inflammation via the MAPK pathways in rabbit articular chondrocytes


Citation

Eo S.H., . and Kim S. J., . Resveratrol-enriched transgenic rice callus extract (IS526) causes inflammation via the MAPK pathways in rabbit articular chondrocytes. pp. 867-874. ISSN 22317546

Abstract

Osteoarthritis is a common degenerative joint disease. Resveratrol-enriched rice (Iksan526) was used in the present work to investigate the effects of IS526 callus extract (IS526) on inflammatory mediators in rabbit articular chondrocytes. Prostaglandin E‚‚ (PGE‚‚) was detected by an assay kit. Protein levels of cyclooxygenase-2 (COX-2) extracellular signal-regulated kinases (ERK)1/2 and p38 kinase were measured by western blotting. IS526 induced the expression of COX-2 and PGE‚‚ . In addition after treatment with IS526 both p38 and ERK1/2 were phosphorylated. Inhibiting ERK1/2 and p38 kinase with PD98059 and SB203580 suppressed IS526-stimulated PGE‚‚ and expression of COX-2 respectively. These findings suggest that IS526 induces inflammation via the p38 kinase and ERK1/2 pathways in rabbit articular chondrocytes.


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Abstract

Osteoarthritis is a common degenerative joint disease. Resveratrol-enriched rice (Iksan526) was used in the present work to investigate the effects of IS526 callus extract (IS526) on inflammatory mediators in rabbit articular chondrocytes. Prostaglandin E‚‚ (PGE‚‚) was detected by an assay kit. Protein levels of cyclooxygenase-2 (COX-2) extracellular signal-regulated kinases (ERK)1/2 and p38 kinase were measured by western blotting. IS526 induced the expression of COX-2 and PGE‚‚ . In addition after treatment with IS526 both p38 and ERK1/2 were phosphorylated. Inhibiting ERK1/2 and p38 kinase with PD98059 and SB203580 suppressed IS526-stimulated PGE‚‚ and expression of COX-2 respectively. These findings suggest that IS526 induces inflammation via the p38 kinase and ERK1/2 pathways in rabbit articular chondrocytes.

Additional Metadata

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Item Type: Article
AGROVOC Term: Rabbits
AGROVOC Term: Rice
AGROVOC Term: Transgenic plants
AGROVOC Term: Joint diseases
AGROVOC Term: Biochemical pathways
AGROVOC Term: Inflammation
AGROVOC Term: Immunofluorescence
AGROVOC Term: Plant extracts
AGROVOC Term: Western blot analysis
AGROVOC Term: Protein kinase
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/9406

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